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Literature summary for 1.3.3.5 extracted from

  • Dos Santos, L.; Climent, V.; Blanford, C.F.; Armstrong, F.A.
    Mechanistic studies of the blue Cu enzyme, bilirubin oxidase, as a highly efficient electrocatalyst for the oxygen reduction reaction (2010), Phys. Chem. Chem. Phys., 12, 13962-13974.
    View publication on PubMed
Search for more literature for 1.3.3.5

Protein Variants

Protein Variants Comment Organism
additional information attachment of the purified enzyme to a PGE surface, from pyrolytic graphite plates, modified with 6-amino-2-naphthoic acid, modifiers tested in increasing activity are 4-aminothiophenol, 4-aminobenzonitrile, 2-aminoacridine, 2-aminoanthracene, 1-aminoanthracene, 2-aminochrysene, 4-benzyloxyaniline, 4-aminobenzoic acid, 4-aminophenylacetic acid, 6-amino-2-naphthoic acid, the latter giving best results. Method optimization, cyclic voltammetry measuring the electrocatalytic reduction of dioxygen by platinum electrodeposited on PGE, detailed overview Albifimbria verrucaria

Inhibitors

Inhibitors Comment Organism Structure
Cl-
-
 Albifimbria verrucaria

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+
-
 Albifimbria verrucaria

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64200
-
-
 Albifimbria verrucaria

Organism

Organism UniProt Comment Textmining
Albifimbria verrucaria
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by anion exchange chromatography and hydrophobic interaction chromatography Albifimbria verrucaria

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25.4
-
 purified enzyme with substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), pH 6.0, 24°C Albifimbria verrucaria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 i.e. ABTS Albifimbria verrucaria ?
-
 ?

Subunits

Subunits Comment Organism
? x * 64200 Albifimbria verrucaria

Synonyms

Synonyms Comment Organism
blue Cu enzyme
-
 Albifimbria verrucaria

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24
-
 assay at, substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Albifimbria verrucaria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at, substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Albifimbria verrucaria