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Literature summary for 1.3.3.5 extracted from

  • Koval, T.; Svecova, L.; Ostergaard, L.H.; Skalova, T.; Duskova, J.; Hasek, J.; Kolenko, P.; Fejfarova, K.; Stransky, J.; Trundova, M.; Dohnalek, J.
    Trp-His covalent adduct in bilirubin oxidase is crucial for effective bilirubin binding but has a minor role in electron transfer (2019), Sci. Rep., 9, 13700 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Aspergillus oryzae Albifimbria verrucaria

Crystallization (Commentary)

Crystallization (Comment) Organism
wild type and mutant enzymes W396A and W396F bound to ferricyanide, hanging drop vapor diffusion method, using 0.1 M succinic acid, 14% (w/v) polyethylene glycol 3350 Albifimbria verrucaria

Protein Variants

Protein Variants Comment Organism
W396A the mutant shows increased activity with bilirubin, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) or ferricyanide compared to the wild type enzyme Albifimbria verrucaria
W396D the mutant shows practically zero activity compared to the wild type enzyme Albifimbria verrucaria
W396F the mutant shows decreased activity with bilirubin and increased activity with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) or ferricyanide compared to the wild type enzyme Albifimbria verrucaria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 4.0 and 27°C Albifimbria verrucaria
1.2
-
ferrocyanide mutant enzyme W396A, at pH 6.0 and 27°C Albifimbria verrucaria
1.2
-
ferrocyanide wild type enzyme, at pH 6.0 and 27°C Albifimbria verrucaria
1.5
-
ferrocyanide mutant enzyme W396F, at pH 6.0 and 27°C Albifimbria verrucaria
3.1
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W396A, at pH 4.0 and 27°C Albifimbria verrucaria
6.8
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W396F, at pH 4.0 and 27°C Albifimbria verrucaria
20.1
-
phenol 2,6-dimethoxyphenol wild type enzyme, at pH 6.8 and 27°C Albifimbria verrucaria

Metals/Ions

Metals/Ions Comment Organism Structure
Cu the enzyme consists of 3 cupredoxin-like domains with 4 copper ions forming two active sites Albifimbria verrucaria

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 bilirubin + O2 Albifimbria verrucaria
-
2 biliverdin + H2O
-
?
2 bilirubin + O2 Albifimbria verrucaria ATCC24571
-
2 biliverdin + H2O
-
?

Organism

Organism UniProt Comment Textmining
Albifimbria verrucaria Q12737
-
-
Albifimbria verrucaria ATCC24571 Q12737
-
-

Purification (Commentary)

Purification (Comment) Organism
Q sepharose column chromatography Albifimbria verrucaria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 bilirubin + O2
-
Albifimbria verrucaria 2 biliverdin + H2O
-
?
2 bilirubin + O2
-
Albifimbria verrucaria ATCC24571 2 biliverdin + H2O
-
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
Albifimbria verrucaria ?
-
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
Albifimbria verrucaria ATCC24571 ?
-
?
ferrocyanide + O2
-
Albifimbria verrucaria ferricyanide + H2O
-
?
ferrocyanide + O2
-
Albifimbria verrucaria ATCC24571 ferricyanide + H2O
-
?
phenol 2,6-dimethoxyphenol + O2
-
Albifimbria verrucaria ?
-
?
phenol 2,6-dimethoxyphenol + O2
-
Albifimbria verrucaria ATCC24571 ?
-
?

Synonyms

Synonyms Comment Organism
BOX
-
Albifimbria verrucaria

pI Value

Organism Comment pI Value Maximum pI Value
Albifimbria verrucaria isoelectric focusing
-
5