Cloned (Comment) | Organism |
---|---|
expressed in Aspergillus oryzae | Albifimbria verrucaria |
Crystallization (Comment) | Organism |
---|---|
wild type and mutant enzymes W396A and W396F bound to ferricyanide, hanging drop vapor diffusion method, using 0.1 M succinic acid, 14% (w/v) polyethylene glycol 3350 | Albifimbria verrucaria |
Protein Variants | Comment | Organism |
---|---|---|
W396A | the mutant shows increased activity with bilirubin, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) or ferricyanide compared to the wild type enzyme | Albifimbria verrucaria |
W396D | the mutant shows practically zero activity compared to the wild type enzyme | Albifimbria verrucaria |
W396F | the mutant shows decreased activity with bilirubin and increased activity with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) or ferricyanide compared to the wild type enzyme | Albifimbria verrucaria |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild type enzyme, at pH 4.0 and 27°C | Albifimbria verrucaria | |
1.2 | - |
ferrocyanide | mutant enzyme W396A, at pH 6.0 and 27°C | Albifimbria verrucaria | |
1.2 | - |
ferrocyanide | wild type enzyme, at pH 6.0 and 27°C | Albifimbria verrucaria | |
1.5 | - |
ferrocyanide | mutant enzyme W396F, at pH 6.0 and 27°C | Albifimbria verrucaria | |
3.1 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W396A, at pH 4.0 and 27°C | Albifimbria verrucaria | |
6.8 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme W396F, at pH 4.0 and 27°C | Albifimbria verrucaria | |
20.1 | - |
phenol 2,6-dimethoxyphenol | wild type enzyme, at pH 6.8 and 27°C | Albifimbria verrucaria |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu | the enzyme consists of 3 cupredoxin-like domains with 4 copper ions forming two active sites | Albifimbria verrucaria |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 bilirubin + O2 | Albifimbria verrucaria | - |
2 biliverdin + H2O | - |
? | |
2 bilirubin + O2 | Albifimbria verrucaria ATCC24571 | - |
2 biliverdin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Albifimbria verrucaria | Q12737 | - |
- |
Albifimbria verrucaria ATCC24571 | Q12737 | - |
- |
Purification (Comment) | Organism |
---|---|
Q sepharose column chromatography | Albifimbria verrucaria |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 bilirubin + O2 | - |
Albifimbria verrucaria | 2 biliverdin + H2O | - |
? | |
2 bilirubin + O2 | - |
Albifimbria verrucaria ATCC24571 | 2 biliverdin + H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Albifimbria verrucaria | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Albifimbria verrucaria ATCC24571 | ? | - |
? | |
ferrocyanide + O2 | - |
Albifimbria verrucaria | ferricyanide + H2O | - |
? | |
ferrocyanide + O2 | - |
Albifimbria verrucaria ATCC24571 | ferricyanide + H2O | - |
? | |
phenol 2,6-dimethoxyphenol + O2 | - |
Albifimbria verrucaria | ? | - |
? | |
phenol 2,6-dimethoxyphenol + O2 | - |
Albifimbria verrucaria ATCC24571 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BOX | - |
Albifimbria verrucaria |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Albifimbria verrucaria | isoelectric focusing | - |
5 |