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Literature summary for 1.3.3.6 extracted from
- Intrinsic enoyl-CoA isomerase activity of rat acyl-CoA oxidase I (2006), Biochim. Biophys. Acta, 1760, 78-85.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | isomerase activity of rat peroxisomal acyl-CoA oxidase I, is probably due to a spontaneous process driven by thermodynamic equilibrium with formation of a conjugated structure after deprotonation of substrate alpha-proton | Rattus norvegicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tagged | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E421A | does not show any isomerase activity | Rattus norvegicus |
| E421D | isomerase activity is decreased for all tested cis- and trans-substrates compared with that of wild-type enzyme | Rattus norvegicus |
| E421Q | does not show any isomerase activity | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.044 | - |
cis-3-decenoyl-CoA | mutant E421D | Rattus norvegicus |  |
| 0.054 | - |
cis-3-decenoyl-CoA | wild-type | Rattus norvegicus | |
| 0.054 | - |
cis-3-octenoyl-CoA | mutant E421D | Rattus norvegicus | |
| 0.056 | - |
trans-3-decenoyl-CoA | wild-type | Rattus norvegicus | |
| 0.057 | - |
cis-3-octenoyl-CoA | wild-type | Rattus norvegicus | |
| 0.059 | - |
trans-3-octenoyl-CoA | wild-type | Rattus norvegicus | |
| 0.063 | - |
trans-3-hexenoyl-CoA | wild-type | Rattus norvegicus | |
| 0.063 | - |
cis-3-hexenoyl-CoA | wild-type | Rattus norvegicus | |
| 0.064 | - |
trans-3-decenoyl-CoA | mutant E421D | Rattus norvegicus | |
| 0.066 | - |
trans-3-octenoyl-CoA | mutant E421D | Rattus norvegicus | |
| 0.076 | - |
cis-3-hexenoyl-CoA | mutant E421D | Rattus norvegicus | |
| 0.083 | - |
trans-3-hexenoyl-CoA | mutant E421D | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peroxisome | - |
Rattus norvegicus | 5777 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-3-decenoyl-CoA + O2 | - |
Rattus norvegicus | ? | - |
? | |
| cis-3-hexenoyl-CoA + O2 | best substrate for the isomerase activity of the enzyme | Rattus norvegicus | ? | - |
? | |
| cis-3-octenoyl-CoA + O2 | - |
Rattus norvegicus | ? | - |
? | |
| trans-3-decenoyl-CoA + O2 | - |
Rattus norvegicus | ? | - |
? | |
| trans-3-hexenoyl-CoA + O2 | - |
Rattus norvegicus | ? | - |
? | |
| trans-3-octenoyl-CoA + O2 | - |
Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACO I | - |
Rattus norvegicus |
| acyl-CoA oxidase I | - |
Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0053 | - |
trans-3-decenoyl-CoA | mutant E421D | Rattus norvegicus | |
| 0.012 | - |
trans-3-octenoyl-CoA | mutant E421D | Rattus norvegicus | |
| 0.01567 | - |
trans-3-decenoyl-CoA | wild-type | Rattus norvegicus | |
| 0.0283 | - |
trans-3-hexenoyl-CoA | mutant E421D | Rattus norvegicus | |
| 0.04 | - |
cis-3-decenoyl-CoA | mutant E421D | Rattus norvegicus | |
| 0.045 | - |
trans-3-octenoyl-CoA | wild-type | Rattus norvegicus | |
| 0.05 | - |
trans-3-hexenoyl-CoA | wild-type | Rattus norvegicus | |
| 0.053 | - |
cis-3-octenoyl-CoA | mutant E421D | Rattus norvegicus | |
| 0.06167 | - |
cis-3-decenoyl-CoA | wild-type | Rattus norvegicus | |
| 0.075 | - |
cis-3-octenoyl-CoA | wild-type | Rattus norvegicus | |
| 0.165 | - |
cis-3-hexenoyl-CoA | mutant E421D | Rattus norvegicus | |
| 0.35 | - |
cis-3-hexenoyl-CoA | wild-type | Rattus norvegicus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | required for the isomerase activity of the enzyme | Rattus norvegicus | |
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