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Literature summary for 1.3.3.6 extracted from

  • Deng, S.; Li, P.; Wang, Y.; Zeng, J.
    tyrosine residues 232 and 401 play a critical role in the binding of the cofactor FAD of acyl-CoA oxidase (2018), Appl. Biochem. Biotechnol., 185, 875-883 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
Y232F the mutant shows reduced activity compared to the wild type enzyme Rattus norvegicus
Y232G the mutant shows reduced activity compared to the wild type enzyme Rattus norvegicus
Y232S the mutant shows reduced activity compared to the wild type enzyme Rattus norvegicus
Y401F the mutant shows reduced activity compared to the wild type enzyme Rattus norvegicus
Y401G the inactive mutant shows no FAD binding Rattus norvegicus
Y401S the inactive mutant shows no FAD binding Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
octanoyl-CoA mutant enzyme Y232G, at pH 7.4 and 30°C Rattus norvegicus
0.0443
-
octanoyl-CoA wild type enzyme, at pH 7.4 and 30°C Rattus norvegicus
0.0502
-
octanoyl-CoA mutant enzyme Y401F, at pH 7.4 and 30°C Rattus norvegicus
0.0573
-
octanoyl-CoA mutant enzyme Y232S, at pH 7.4 and 30°C Rattus norvegicus
0.0612
-
octanoyl-CoA mutant enzyme Y232F, at pH 7.4 and 30°C Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P07872
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap chelating metal-affinity column chromatography Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
octanoyl-CoA + O2
-
Rattus norvegicus trans-2,3-dehydrooctanoyl-CoA + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 66200, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
ACO
-
Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.054
-
octanoyl-CoA mutant enzyme Y401F, at pH 7.4 and 30°C Rattus norvegicus
0.056
-
octanoyl-CoA mutant enzyme Y232G, at pH 7.4 and 30°C Rattus norvegicus
0.3
-
octanoyl-CoA mutant enzyme Y232F, at pH 7.4 and 30°C Rattus norvegicus
0.5
-
octanoyl-CoA mutant enzyme Y232S, at pH 7.4 and 30°C Rattus norvegicus
3.47
-
octanoyl-CoA wild type enzyme, at pH 7.4 and 30°C Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Rattus norvegicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1
-
octanoyl-CoA mutant enzyme Y401F, at pH 7.4 and 30°C Rattus norvegicus
1.3
-
octanoyl-CoA mutant enzyme Y232G, at pH 7.4 and 30°C Rattus norvegicus
4.9
-
octanoyl-CoA mutant enzyme Y232F, at pH 7.4 and 30°C Rattus norvegicus
8.7
-
octanoyl-CoA mutant enzyme Y232S, at pH 7.4 and 30°C Rattus norvegicus
78.3
-
octanoyl-CoA wild type enzyme, at pH 7.4 and 30°C Rattus norvegicus