Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
Y232F | the mutant shows reduced activity compared to the wild type enzyme | Rattus norvegicus |
Y232G | the mutant shows reduced activity compared to the wild type enzyme | Rattus norvegicus |
Y232S | the mutant shows reduced activity compared to the wild type enzyme | Rattus norvegicus |
Y401F | the mutant shows reduced activity compared to the wild type enzyme | Rattus norvegicus |
Y401G | the inactive mutant shows no FAD binding | Rattus norvegicus |
Y401S | the inactive mutant shows no FAD binding | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
octanoyl-CoA | mutant enzyme Y232G, at pH 7.4 and 30°C | Rattus norvegicus | |
0.0443 | - |
octanoyl-CoA | wild type enzyme, at pH 7.4 and 30°C | Rattus norvegicus | |
0.0502 | - |
octanoyl-CoA | mutant enzyme Y401F, at pH 7.4 and 30°C | Rattus norvegicus | |
0.0573 | - |
octanoyl-CoA | mutant enzyme Y232S, at pH 7.4 and 30°C | Rattus norvegicus | |
0.0612 | - |
octanoyl-CoA | mutant enzyme Y232F, at pH 7.4 and 30°C | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P07872 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap chelating metal-affinity column chromatography | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
octanoyl-CoA + O2 | - |
Rattus norvegicus | trans-2,3-dehydrooctanoyl-CoA + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66200, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
ACO | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
octanoyl-CoA | mutant enzyme Y401F, at pH 7.4 and 30°C | Rattus norvegicus | |
0.056 | - |
octanoyl-CoA | mutant enzyme Y232G, at pH 7.4 and 30°C | Rattus norvegicus | |
0.3 | - |
octanoyl-CoA | mutant enzyme Y232F, at pH 7.4 and 30°C | Rattus norvegicus | |
0.5 | - |
octanoyl-CoA | mutant enzyme Y232S, at pH 7.4 and 30°C | Rattus norvegicus | |
3.47 | - |
octanoyl-CoA | wild type enzyme, at pH 7.4 and 30°C | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rattus norvegicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
octanoyl-CoA | mutant enzyme Y401F, at pH 7.4 and 30°C | Rattus norvegicus | |
1.3 | - |
octanoyl-CoA | mutant enzyme Y232G, at pH 7.4 and 30°C | Rattus norvegicus | |
4.9 | - |
octanoyl-CoA | mutant enzyme Y232F, at pH 7.4 and 30°C | Rattus norvegicus | |
8.7 | - |
octanoyl-CoA | mutant enzyme Y232S, at pH 7.4 and 30°C | Rattus norvegicus | |
78.3 | - |
octanoyl-CoA | wild type enzyme, at pH 7.4 and 30°C | Rattus norvegicus |