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Literature summary for 1.3.3.7 extracted from
- Partial purification and characterization of dihydrouracil oxidase, a flavoprotein from Rhodotorula glutinis (1986), J. Ferment. Technol., 64, 205-210.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-Bromouracil | competitive vs. dihydrouracil | Rhodotorula glutinis |  |
| 5-Chlorouracil | competitive vs. dihydrouracil | Rhodotorula glutinis | |
| 5-fluorouracil | competitive vs. dihydrouracil | Rhodotorula glutinis | |
| 5-Iodouracil | competitive vs. dihydrouracil | Rhodotorula glutinis | |
| 5-Nitrouracil | competitive vs. dihydrouracil | Rhodotorula glutinis | |
| p-chloromercuribenzoate | - |
Rhodotorula glutinis | |
| thymine | competitive vs. dihydrouracil | Rhodotorula glutinis | |
| Uracil | competitive vs. dihydrouracil | Rhodotorula glutinis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
dihydrouracil | - |
Rhodotorula glutinis | |
| 0.05 | - |
Dihydrothymine | - |
Rhodotorula glutinis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
gel filtration | Rhodotorula glutinis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula glutinis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-cellulose, DEAE-Sephadex a-50, electrofocusing, Sephadex G-200, partially purified | Rhodotorula glutinis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.25 | - |
- |
Rhodotorula glutinis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil + O2 | O2 is the only known electron acceptor, no substrates are dihydro-6-methyluracil, dihydro D-orotic acid and dihydro L-orotic acid | Rhodotorula glutinis | uracil + H2O2 | - |
? | |
| dihydrothymine + O2 | - |
Rhodotorula glutinis | thymine + H2O2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Rhodotorula glutinis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable up to | Rhodotorula glutinis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Rhodotorula glutinis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | 1 h, 25°C, stable | Rhodotorula glutinis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | flavoprotein | Rhodotorula glutinis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
thymine | - |
Rhodotorula glutinis | |
| 0.0035 | - |
5-Nitrouracil | - |
Rhodotorula glutinis | |
| 0.01 | - |
5-fluorouracil | - |
Rhodotorula glutinis | |
| 0.02 | - |
5-Chlorouracil | - |
Rhodotorula glutinis | |
| 0.032 | - |
5-Bromouracil | - |
Rhodotorula glutinis | |
| 0.035 | - |
Uracil | - |
Rhodotorula glutinis | |
| 0.088 | - |
5-Iodouracil | - |
Rhodotorula glutinis | |
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