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Literature summary for 1.3.3.7 extracted from

  • Owaki, J.; Uzura, K.; Minami, Z.; Kuai, K.
    Partial purification and characterization of dihydrouracil oxidase, a flavoprotein from Rhodotorula glutinis (1986), J. Ferment. Technol., 64, 205-210.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
5-Bromouracil competitive vs. dihydrouracil Rhodotorula glutinis
5-Chlorouracil competitive vs. dihydrouracil Rhodotorula glutinis
5-fluorouracil competitive vs. dihydrouracil Rhodotorula glutinis
5-Iodouracil competitive vs. dihydrouracil Rhodotorula glutinis
5-Nitrouracil competitive vs. dihydrouracil Rhodotorula glutinis
p-chloromercuribenzoate
-
Rhodotorula glutinis
thymine competitive vs. dihydrouracil Rhodotorula glutinis
Uracil competitive vs. dihydrouracil Rhodotorula glutinis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
dihydrouracil
-
Rhodotorula glutinis
0.05
-
Dihydrothymine
-
Rhodotorula glutinis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
gel filtration Rhodotorula glutinis

Organism

Organism UniProt Comment Textmining
Rhodotorula glutinis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-cellulose, DEAE-Sephadex a-50, electrofocusing, Sephadex G-200, partially purified Rhodotorula glutinis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
9.25
-
-
Rhodotorula glutinis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6-dihydrouracil + O2 O2 is the only known electron acceptor, no substrates are dihydro-6-methyluracil, dihydro D-orotic acid and dihydro L-orotic acid Rhodotorula glutinis uracil + H2O2
-
?
dihydrothymine + O2
-
Rhodotorula glutinis thymine + H2O2
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
7 8
-
Rhodotorula glutinis

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
30
-
stable up to Rhodotorula glutinis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Rhodotorula glutinis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 1 h, 25┬░C, stable Rhodotorula glutinis

Cofactor

Cofactor Comment Organism Structure
FMN flavoprotein Rhodotorula glutinis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.003
-
thymine
-
Rhodotorula glutinis
0.0035
-
5-Nitrouracil
-
Rhodotorula glutinis
0.01
-
5-fluorouracil
-
Rhodotorula glutinis
0.02
-
5-Chlorouracil
-
Rhodotorula glutinis
0.032
-
5-Bromouracil
-
Rhodotorula glutinis
0.035
-
Uracil
-
Rhodotorula glutinis
0.088
-
5-Iodouracil
-
Rhodotorula glutinis