Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.4.1 extracted from

  • Khandekar, S.S.; Eirich, L.D.
    Purification and characterization of an anabolic fumarate reductase from Methanobacterium thermoautotrophicum (1989), Appl. Environ. Microbiol., 55, 856-861.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
CuCl2 5 mM, about 80% loss of activity Methanothermobacter thermautotrophicus
iodoacetamide 1 mM, about 80% loss of activity after 20 min Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
fumarate pH 7.0, 75°C Methanothermobacter thermautotrophicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Methanothermobacter thermautotrophicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
x * 20000, SDS-PAGE Methanothermobacter thermautotrophicus
78000
-
gel filtration Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fumarate + CoM + CoB Methanothermobacter thermautotrophicus
-
succinate + CoM-S-S-CoB
-
?
fumarate + CoM + CoB Methanothermobacter thermautotrophicus DELTAH
-
succinate + CoM-S-S-CoB
-
?

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-
Methanothermobacter thermautotrophicus DELTAH
-
-
-

Oxidation Stability

Oxidation Stability Organism
very oxygen sensitive Methanothermobacter thermautotrophicus

Purification (Commentary)

Purification (Comment) Organism
-
Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fumarate + CoM + CoB
-
Methanothermobacter thermautotrophicus succinate + CoM-S-S-CoB
-
?
fumarate + CoM + CoB the enzyme cannot use reduced flavins, reduced coenzyme F420, or NAD(P)H as an electron donor Methanothermobacter thermautotrophicus succinate + CoM-S-S-CoB
-
?
fumarate + CoM + CoB
-
Methanothermobacter thermautotrophicus DELTAH succinate + CoM-S-S-CoB
-
?
fumarate + CoM + CoB the enzyme cannot use reduced flavins, reduced coenzyme F420, or NAD(P)H as an electron donor Methanothermobacter thermautotrophicus DELTAH succinate + CoM-S-S-CoB
-
?

Subunits

Subunits Comment Organism
? x * 20000, SDS-PAGE Methanothermobacter thermautotrophicus

Synonyms

Synonyms Comment Organism
Tfr
-
Methanothermobacter thermautotrophicus
thiol:fumarate reductase
-
Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Methanothermobacter thermautotrophicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 40°C: about 40% of maximal activity, 80°C: about 50% of maximal activity Methanothermobacter thermautotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
no detectable loss in the activity is observed when the enzyme (0.03 mg of protein per ml) is incubated anaerobically at 70°C in sodium phosphate buffer (pH 7.0) containing 1 mM dithiothreitol and 15 mM 2-mercaptoethanol for 8 h Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Methanothermobacter thermautotrophicus

Cofactor

Cofactor Comment Organism Structure
additional information absence of flavins as a cofactor. The spectral data, suggests the presence of an unknown cofactor tightly bound to the enzyme Methanothermobacter thermautotrophicus