Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuCl2 | 5 mM, about 80% loss of activity | Methanothermobacter thermautotrophicus | |
iodoacetamide | 1 mM, about 80% loss of activity after 20 min | Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
fumarate | pH 7.0, 75°C | Methanothermobacter thermautotrophicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Methanothermobacter thermautotrophicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
x * 20000, SDS-PAGE | Methanothermobacter thermautotrophicus |
78000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
fumarate + CoM + CoB | Methanothermobacter thermautotrophicus | - |
succinate + CoM-S-S-CoB | - |
? | |
fumarate + CoM + CoB | Methanothermobacter thermautotrophicus DELTAH | - |
succinate + CoM-S-S-CoB | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Methanothermobacter thermautotrophicus DELTAH | - |
- |
- |
Oxidation Stability | Organism |
---|---|
very oxygen sensitive | Methanothermobacter thermautotrophicus |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fumarate + CoM + CoB | - |
Methanothermobacter thermautotrophicus | succinate + CoM-S-S-CoB | - |
? | |
fumarate + CoM + CoB | the enzyme cannot use reduced flavins, reduced coenzyme F420, or NAD(P)H as an electron donor | Methanothermobacter thermautotrophicus | succinate + CoM-S-S-CoB | - |
? | |
fumarate + CoM + CoB | - |
Methanothermobacter thermautotrophicus DELTAH | succinate + CoM-S-S-CoB | - |
? | |
fumarate + CoM + CoB | the enzyme cannot use reduced flavins, reduced coenzyme F420, or NAD(P)H as an electron donor | Methanothermobacter thermautotrophicus DELTAH | succinate + CoM-S-S-CoB | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 20000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Synonyms | Comment | Organism |
---|---|---|
Tfr | - |
Methanothermobacter thermautotrophicus |
thiol:fumarate reductase | - |
Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Methanothermobacter thermautotrophicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | 40°C: about 40% of maximal activity, 80°C: about 50% of maximal activity | Methanothermobacter thermautotrophicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
no detectable loss in the activity is observed when the enzyme (0.03 mg of protein per ml) is incubated anaerobically at 70°C in sodium phosphate buffer (pH 7.0) containing 1 mM dithiothreitol and 15 mM 2-mercaptoethanol for 8 h | Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Methanothermobacter thermautotrophicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | absence of flavins as a cofactor. The spectral data, suggests the presence of an unknown cofactor tightly bound to the enzyme | Methanothermobacter thermautotrophicus |