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show all sequences of 1.3.4.1

Purification and characterization of an anabolic fumarate reductase from Methanobacterium thermoautotrophicum

Khandekar, S.S.; Eirich, L.D.; Appl. Environ. Microbiol. 55, 856-861 (1989)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CuCl2
5 mM, about 80% loss of activity
Methanothermobacter thermautotrophicus
iodoacetamide
1 mM, about 80% loss of activity after 20 min
Methanothermobacter thermautotrophicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
fumarate
pH 7.0, 75°C
Methanothermobacter thermautotrophicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Methanothermobacter thermautotrophicus
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
x * 20000, SDS-PAGE
Methanothermobacter thermautotrophicus
78000
-
gel filtration
Methanothermobacter thermautotrophicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
fumarate + CoM + CoB
Methanothermobacter thermautotrophicus
-
succinate + CoM-S-S-CoB
-
-
?
fumarate + CoM + CoB
Methanothermobacter thermautotrophicus DELTAH
-
succinate + CoM-S-S-CoB
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanothermobacter thermautotrophicus
-
-
-
Methanothermobacter thermautotrophicus DELTAH
-
-
-
Oxidation Stability
Oxidation Stability
Organism
very oxygen sensitive
Methanothermobacter thermautotrophicus
Purification (Commentary)
Commentary
Organism
-
Methanothermobacter thermautotrophicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
fumarate + CoM + CoB
-
723990
Methanothermobacter thermautotrophicus
succinate + CoM-S-S-CoB
-
-
-
?
fumarate + CoM + CoB
the enzyme cannot use reduced flavins, reduced coenzyme F420, or NAD(P)H as an electron donor
723990
Methanothermobacter thermautotrophicus
succinate + CoM-S-S-CoB
-
-
-
?
fumarate + CoM + CoB
-
723990
Methanothermobacter thermautotrophicus DELTAH
succinate + CoM-S-S-CoB
-
-
-
?
fumarate + CoM + CoB
the enzyme cannot use reduced flavins, reduced coenzyme F420, or NAD(P)H as an electron donor
723990
Methanothermobacter thermautotrophicus DELTAH
succinate + CoM-S-S-CoB
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 20000, SDS-PAGE
Methanothermobacter thermautotrophicus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
75
-
-
Methanothermobacter thermautotrophicus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
40
80
40°C: about 40% of maximal activity, 80°C: about 50% of maximal activity
Methanothermobacter thermautotrophicus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
no detectable loss in the activity is observed when the enzyme (0.03 mg of protein per ml) is incubated anaerobically at 70°C in sodium phosphate buffer (pH 7.0) containing 1 mM dithiothreitol and 15 mM 2-mercaptoethanol for 8 h
Methanothermobacter thermautotrophicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Methanothermobacter thermautotrophicus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
absence of flavins as a cofactor. The spectral data, suggests the presence of an unknown cofactor tightly bound to the enzyme
Methanothermobacter thermautotrophicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
absence of flavins as a cofactor. The spectral data, suggests the presence of an unknown cofactor tightly bound to the enzyme
Methanothermobacter thermautotrophicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CuCl2
5 mM, about 80% loss of activity
Methanothermobacter thermautotrophicus
iodoacetamide
1 mM, about 80% loss of activity after 20 min
Methanothermobacter thermautotrophicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
fumarate
pH 7.0, 75°C
Methanothermobacter thermautotrophicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Methanothermobacter thermautotrophicus
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
x * 20000, SDS-PAGE
Methanothermobacter thermautotrophicus
78000
-
gel filtration
Methanothermobacter thermautotrophicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
fumarate + CoM + CoB
Methanothermobacter thermautotrophicus
-
succinate + CoM-S-S-CoB
-
-
?
fumarate + CoM + CoB
Methanothermobacter thermautotrophicus DELTAH
-
succinate + CoM-S-S-CoB
-
-
?
Oxidation Stability (protein specific)
Oxidation Stability
Organism
very oxygen sensitive
Methanothermobacter thermautotrophicus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methanothermobacter thermautotrophicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
fumarate + CoM + CoB
-
723990
Methanothermobacter thermautotrophicus
succinate + CoM-S-S-CoB
-
-
-
?
fumarate + CoM + CoB
the enzyme cannot use reduced flavins, reduced coenzyme F420, or NAD(P)H as an electron donor
723990
Methanothermobacter thermautotrophicus
succinate + CoM-S-S-CoB
-
-
-
?
fumarate + CoM + CoB
-
723990
Methanothermobacter thermautotrophicus DELTAH
succinate + CoM-S-S-CoB
-
-
-
?
fumarate + CoM + CoB
the enzyme cannot use reduced flavins, reduced coenzyme F420, or NAD(P)H as an electron donor
723990
Methanothermobacter thermautotrophicus DELTAH
succinate + CoM-S-S-CoB
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 20000, SDS-PAGE
Methanothermobacter thermautotrophicus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
75
-
-
Methanothermobacter thermautotrophicus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
40
80
40°C: about 40% of maximal activity, 80°C: about 50% of maximal activity
Methanothermobacter thermautotrophicus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
no detectable loss in the activity is observed when the enzyme (0.03 mg of protein per ml) is incubated anaerobically at 70°C in sodium phosphate buffer (pH 7.0) containing 1 mM dithiothreitol and 15 mM 2-mercaptoethanol for 8 h
Methanothermobacter thermautotrophicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Methanothermobacter thermautotrophicus
Other publictions for EC 1.3.4.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723990
Khandekar
Purification and characterizat ...
Methanothermobacter thermautotrophicus, Methanothermobacter thermautotrophicus DELTAH
Appl. Environ. Microbiol.
55
856-861
1989
-
-
-
-
-
-
2
1
1
-
2
2
-
14
1
-
1
-
-
-
-
-
4
1
1
1
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
1
1
-
2
2
-
1
-
1
-
-
-
-
4
1
1
1
1
-
1
-
-
-
-
-
-
-
-
-