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Literature summary for 1.3.5.1 extracted from

  • Yang, X.; Yu, L.; Yu, C.A.
    Resolution and reconstitution of succinate-ubiquinone reductase from Escherichia coli (1997), J. Biol. Chem., 272, 9683-9689.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-Thenoyltrifluoroacetone 50% inhibition at 3 mM Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13000
-
1 * 64000 + 1 * 28000 + 1+ 14000 + 1 * 13000, SDS-PAGE Escherichia coli
28000
-
1 * 64000 + 1 * 28000 + 1+ 14000 + 1 * 13000, SDS-PAGE Escherichia coli
64000
-
1 * 64000 + 1 * 28000 + 1+ 14000 + 1 * 13000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
succinate dehydrogenase
-

Purification (Commentary)

Purification (Comment) Organism
using solubilization with polyoxyethylene-9-lauryl ether and column chromatography on DEAE-Sepharose CL-6B Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
the isolated enzyme is resolved into a reconstitutively active, two-subunit succinate dehydrogenase and a two-subunit membrane anchoring protein fraction by alkaline treatment in the presence of urea followed by DEAE-Sepharose CL-6B column chromatography, maximum reconstitution is obtained when the weight ratio of succinate dehydrogenase to the two-subunit membrane anchoring protein reaches 5.26 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinate + ubiquinone
-
Escherichia coli fumarate + ubiquinol
-
?

Subunits

Subunits Comment Organism
tetramer 1 * 64000 + 1 * 28000 + 1+ 14000 + 1 * 13000, SDS-PAGE Escherichia coli

Cofactor

Cofactor Comment Organism Structure
cytochrome b the isolated two-subunit membrane anchoring protein contains 35 nmol cytochrome b-556 per mg protein Escherichia coli