Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Thenoyltrifluoroacetone | 50% inhibition at 3 mM | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13000 | - |
1 * 64000 + 1 * 28000 + 1+ 14000 + 1 * 13000, SDS-PAGE | Escherichia coli |
28000 | - |
1 * 64000 + 1 * 28000 + 1+ 14000 + 1 * 13000, SDS-PAGE | Escherichia coli |
64000 | - |
1 * 64000 + 1 * 28000 + 1+ 14000 + 1 * 13000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
succinate dehydrogenase | - |
Purification (Comment) | Organism |
---|---|
using solubilization with polyoxyethylene-9-lauryl ether and column chromatography on DEAE-Sepharose CL-6B | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
the isolated enzyme is resolved into a reconstitutively active, two-subunit succinate dehydrogenase and a two-subunit membrane anchoring protein fraction by alkaline treatment in the presence of urea followed by DEAE-Sepharose CL-6B column chromatography, maximum reconstitution is obtained when the weight ratio of succinate dehydrogenase to the two-subunit membrane anchoring protein reaches 5.26 | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinate + ubiquinone | - |
Escherichia coli | fumarate + ubiquinol | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 1 * 64000 + 1 * 28000 + 1+ 14000 + 1 * 13000, SDS-PAGE | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b | the isolated two-subunit membrane anchoring protein contains 35 nmol cytochrome b-556 per mg protein | Escherichia coli |