Any feedback?
Literature summary for 1.3.5.1 extracted from
- The Saccharomyces cerevisiae succinate dehydrogenase does not require heme for ubiquinone reduction (2007), Biochim. Biophys. Acta, 1767, 1436-1445.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| complementation of a commercially available SDH3/SDH4 double knockout mutant yeast strain, subcloning of SDH genes in Escherichia coli strain DH5alpha | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C78A | site-directed mutagenesis of the subunit Sdh4p residue an axial ligand of heme binding, leads to highly reduced to undetectable levels of heme b562 compared to the wild-type enzyme | Saccharomyces cerevisiae |
| C78A/H106A | site-directed mutagenesis of the subunits Sdh3p and Sdh4p, leads to highly reduced to undetectable levels of heme b562 and reduced cell growth compared to the wild-type enzyme | Saccharomyces cerevisiae |
| H106A | site-directed mutagenesis of the subunit Sdh3p residue, an axial ligand of heme binding, leads to highly reduced to undetectable levels of heme b562 compared to the wild-type enzyme | Saccharomyces cerevisiae |
| additional information | construction of a gene SDH3 disruption mutant by gene replacement, effects of mutations of the enzyme subunits on the electron transfer activities in mitochondrial membranes of mutant yeast cells compared to the wild-type strain, overview | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
2,6-dichlorophenol indophenol | 22°C, wild-type strain YPH499 | Saccharomyces cerevisiae |  |
| 0.007 | - |
2,6-dichlorophenol indophenol | 22°C, Sdh3p/Sdh4p double mutant strain H106A/C78A | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial membrane | the enzyme has a heme b-containing membrane-anchoring dimer, comprising the Sdh3p and Sdh4p subunits, overview | Saccharomyces cerevisiae | 31966 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | non-heme iron in the iron-sulfur protein in subunit Sdh2p as part of the catalytic dimer of the tetrameric enzyme, and heme iron in a heme b-containing membrane-anchoring dimer, comprising the Sdh3p and Sdh4p subunits, overview | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinate + ubiquinone | Saccharomyces cerevisiae | - |
fumarate + ubiquinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
wild-type strain YPH499 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinate + 2,6-dichlorophenol indophenol | - |
Saccharomyces cerevisiae | fumarate + reduced 2,6-dichlorophenol indophenol | - |
? | |
| succinate + ubiquinone | - |
Saccharomyces cerevisiae | fumarate + ubiquinol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | structure molecular dynamic simulations using the crystal structure with PDB ID 1PB4 | Saccharomyces cerevisiae |
| tetramer | composed of a catalytic dimer, comprising a flavoprotein subunit Sdh1p and an iron-sulfur protein Sdh2p, and a heme b-containing membrane-anchoring dimer, comprising the Sdh3p and Sdh4p subunits, overview | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SDH | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavoprotein, 41 pmol FAD per mg of protein in wild-type strain YPH499, possesses a flavoprotein subunit Sdh1p as part of the catalytic dimer of the tetrameric enzyme | Saccharomyces cerevisiae | |
| heme b562 | the enzyme has a heme b-containing membrane-anchoring dimer, comprising the Sdh3p and Sdh4p subunits, overview | Saccharomyces cerevisiae | |
| ubiquinone | - |
Saccharomyces cerevisiae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
