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Literature summary for 1.3.5.2 extracted from
- Dihydroorotate dehydrogenase (Neurospora) (1978), Methods Enzymol., 51, 63-69.
General Stability
| General Stability | Organism |
|---|---|
| dihydroorotate stabilizes during purification and dialysis and heat inactivation | Neurospora crassa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fatty acids | noncompetitive with respect to ubiquinone | Neurospora crassa |  |
| Orotate | - |
Neurospora crassa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | membrane | Neurospora crassa | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | 1.4 mol of ferric iron per 120000 g of active enzyme | Neurospora crassa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 210000 | - |
gel filtration after phospholipase treatment | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydroorotate + acceptor | Neurospora crassa | - |
orotate + reduced acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| lipoprotein | contains solvent-extractable fatty acids and phospholipids | Neurospora crassa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neurospora crassa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (S)-dihydroorotate + a quinone = orotate + a quinol | ping-pong mechanism | Neurospora crassa | |
| (S)-dihydroorotate + a quinone = orotate + a quinol | soluble quinones as second substrate | Neurospora crassa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Neurospora crassa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15 | - |
- |
Neurospora crassa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, several days | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydroorotate + acceptor | - |
Neurospora crassa | orotate + reduced acceptor | - |
? | |
| dihydroorotate + acceptor | acceptor: 2,6-dichlorophenolindophenol | Neurospora crassa | orotate + reduced acceptor | - |
? | |
| dihydroorotate + acceptor | acceptor: quinones | Neurospora crassa | orotate + reduced acceptor | - |
? | |
| dihydroorotate + acceptor | not acceptor: oxygen | Neurospora crassa | orotate + reduced acceptor | - |
? | |
| dihydroorotate + acceptor | cytochrome b, cytochrome c, pyridine nucleotides (NAD+, NADP+) cannot serve as electron acceptors | Neurospora crassa | orotate + reduced acceptor | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Neurospora crassa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | 1 mol of FMN per 120000 g of active enzyme | Neurospora crassa | |
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Release 2023.1 (January 2023)
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