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Literature summary for 1.3.5.2 extracted from

  • Hortua Triana, M.A.; Huynh, M.H.; Garavito, M.F.; Fox, B.A.; Bzik, D.J.; Carruthers, V.B.; Loeffler, M.; Zimmermann, B.H.
    Biochemical and molecular characterization of the pyrimidine biosynthetic enzyme dihydroorotate dehydrogenase from Toxoplasma gondii (2012), Mol. Biochem. Parasitol., 184, 71-81.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.5.2

Inhibitors

Inhibitors Comment Organism Structure
2-cyano-3-hydroxy-N-(2',3,3'-trichlorobiphenyl-4-yl)but-2-enamide
-
 Toxoplasma gondii
2-hydroxyethylidene-cyano acetic acid 4-trifluoromethyl anilide A177-1726, active derivative of human inhibitor leflunomide Toxoplasma gondii
additional information not inhibitory: brequinar, atovaquone, N-(3,5-difluoro-4-(trifluoromethyl)phenyl)-5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-amine Toxoplasma gondii
N-(3-chloro-2'-methoxybiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide
-
 Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 ubiquinone-50 pH 8.0, 30°C Toxoplasma gondii
0.028
-
 1,4-Naphthoquinone pH 8.0, 30°C Toxoplasma gondii
0.029
-
 decylubiquinone pH 8.0, 30°C Toxoplasma gondii
0.051
-
 ubiquinone-30 pH 8.0, 30°C Toxoplasma gondii
0.06
-
 (S)-dihydroorotate pH 8.0, 30°C Toxoplasma gondii
0.099
-
 2,3-dimethoxy-5-methyl-1,4-benzoquinone pH 8.0, 30°C Toxoplasma gondii
0.132
-
 2,5-dimethyl-p-benzoquinone pH 8.0, 30°C Toxoplasma gondii

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Toxoplasma gondii 5739
-

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
84
-
 pH 8.0, 30°C Toxoplasma gondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + 1,4-naphthoquinone
-
 Toxoplasma gondii orotate + 1,4-naphthoquinol
-
 ?
(S)-dihydroorotate + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
 Toxoplasma gondii orotate + 2,3-dimethoxy-5-methyl-1,4-benzoquinol
-
 ?
(S)-dihydroorotate + 2,5-dimethyl-p-benzoquinone
-
 Toxoplasma gondii orotate + 2,5-dimethyl-p-benzoquinol
-
 ?
(S)-dihydroorotate + decylubiquinone
-
 Toxoplasma gondii orotate + decylubiquinol
-
 ?
(S)-dihydroorotate + ubiquinone-30
-
 Toxoplasma gondii orotate + ubiquinol-30
-
 ?
(S)-dihydroorotate + ubiquinone-50
-
 Toxoplasma gondii orotate + ubiquinol-50
-
 ?
additional information fumarate is a poor electron acceptor Toxoplasma gondii ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21
-
 ubiquinone-30 pH 8.0, 30°C Toxoplasma gondii
29
-
 1,4-Naphthoquinone pH 8.0, 30°C Toxoplasma gondii
32
-
 2,3-dimethoxy-5-methyl-1,4-benzoquinone pH 8.0, 30°C Toxoplasma gondii
44
-
 2,5-dimethyl-p-benzoquinone pH 8.0, 30°C Toxoplasma gondii
61
-
 ubiquinone-50 pH 8.0, 30°C Toxoplasma gondii
82
-
 (S)-dihydroorotate pH 8.0, 30°C Toxoplasma gondii
89
-
 decylubiquinone pH 8.0, 30°C Toxoplasma gondii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.06
-
 pH 8.0, 30°C Toxoplasma gondii N-(3-chloro-2'-methoxybiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide
0.091
-
 pH 8.0, 30°C Toxoplasma gondii 2-hydroxyethylidene-cyano acetic acid 4-trifluoromethyl anilide
0.096
-
 pH 8.0, 30°C Toxoplasma gondii 2-cyano-3-hydroxy-N-(2',3,3'-trichlorobiphenyl-4-yl)but-2-enamide

General Information

General Information Comment Organism
physiological function the gene appears to be essential, because while gene tagging is successful at the TgDHOD gene locus, attempts to delete the TgDHOD gene were not successful in the KU80 background Toxoplasma gondii