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Literature summary for 1.3.5.5 extracted from

  • Breitenbach, J.; Zhu, C.; Sandmann, G.
    Bleaching herbicide norflurazon inhibits phytoene desaturase by competition with the cofactors (2001), J. Agric. Food Chem., 49, 5270-5272.
    View publication on PubMed
Search for more literature for 1.3.5.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Synechococcus sp.
expression in Escherichia coli Gentiana lutea

Inhibitors

Inhibitors Comment Organism Structure
norflurazon 0.0001 mM, Vmax values are very little influenced by norflurazon, which reveals a competition between NADP+ or plastoquinone and norflurazon for the cofactor binding site of phytoene desaturase Synechococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
15-cis-phytoene + 2 plastoquinone Synechococcus sp.
-
 9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
 ?
15-cis-phytoene + 2 plastoquinone Gentiana lutea
-
 9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
 ?

Organism

Organism UniProt Comment Textmining
Gentiana lutea
-
-
-
Synechococcus sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
15-cis-phytoene + 2 decyl-plastoquinone this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially Synechococcus sp. 9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
 ?
15-cis-phytoene + 2 decyl-plastoquinone this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially. Decyl-plastoquinone is very effective as a cofactor of desaturation. The simultanous presence of NADP+ has no influence on the activity Gentiana lutea 9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
 ?
15-cis-phytoene + 2 decyl-ubiquinone this quinone derivative is used in the study because the natural C45-ubiquinone is not available commercially Synechococcus sp. 9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
 ?
15-cis-phytoene + 2 decylubiquinone this quinone derivative is used in the study because the natural C45-ubiquinone is not available commercially Gentiana lutea 9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
 ?
15-cis-phytoene + 2 plastoquinone
-
 Synechococcus sp. 9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
 ?
15-cis-phytoene + 2 plastoquinone
-
 Gentiana lutea 9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
 ?
additional information no activity with NADP+ Gentiana lutea ?
-
 ?
additional information the enzyme also shows activity with NADP+ as cosubstrate, the affinity for NADP+ is lower than for decylplastoquinone or decylubioquinone Synechococcus sp. ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Synechococcus sp.
28
-
 assay at Gentiana lutea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Synechococcus sp.
7.2
-
 assay at Gentiana lutea

Cofactor

Cofactor Comment Organism Structure
decyl-plastoquinone this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially Gentiana lutea
additional information the enzyme also shows activity with NADP+ as cosubstrate, the affinity for NADP+ is lower than for decylplastoquinone or decylubioquinone Synechococcus sp.
plastoquinone decyl-plastoquinone or decyl-ubiquinone is used in the study because the natural C45-plastoquinone is not available commercially. The affinity of the enzyme for plastoquinone is higher than for NADP+ Synechococcus sp.
Ubiquinone-10 this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially Gentiana lutea