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Literature summary for 1.3.5.5 extracted from

  • Gemmecker, S.; Schaub, P.; Koschmieder, J.; Brausemann, A.; Drepper, F.; Rodriguez-Franco, M.; Ghisla, S.; Warscheid, B.; Einsle, O.; Beyer, P.
    Phytoene desaturase from Oryza sativa oligomeric assembly, membrane association and preliminary 3D-analysis (2015), PLoS ONE, 10, e0131717 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.5.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Oryza sativa Indica Group

Crystallization (Commentary)

Crystallization (Comment) Organism
low-resolution model, homotetrameric arrangement of the protein Oryza sativa Indica Group

Inhibitors

Inhibitors Comment Organism Structure
norflurazon arrests the reaction by stabilizing the intermediary FADred. An excess of the quinone acceptor relieves this blockage Oryza sativa Indica Group

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated to Oryza sativa Indica Group 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
450000
-
 gel filtration Oryza sativa Indica Group

Organism

Organism UniProt Comment Textmining
Oryza sativa Indica Group A2XDA1
-
-

Oxidation Stability

Oxidation Stability Organism
reduced form of protein is stable for over 2 h in ambient oxygen atmosphere, rapid reoxidation can be achieved with excess decyl-plastoquinone Oryza sativa Indica Group

Purification (Commentary)

Purification (Comment) Organism
recombinant protein. High-order oligomers formed in solution are the basis for an active preparation Oryza sativa Indica Group

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
15-cis-phytoene + 2 decyl-plastoquinone
-
 Oryza sativa Indica Group 9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
 ?
additional information benzoquinone cosubstrates are strongly preferred over naphthoquinones Oryza sativa Indica Group ?
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 56200, calculated, 8 * 56000, SDS-PAGE, recombinant protein with His-tag Oryza sativa Indica Group

Synonyms

Synonyms Comment Organism
PDS1
-
 Oryza sativa Indica Group

Cofactor

Cofactor Comment Organism Structure
FAD sole protein-bound redox-cofactor Oryza sativa Indica Group