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Literature summary for 1.3.5.6 extracted from
- Structural and kinetics properties of a mutated phytoene desaturase from Rubrivivax gelatinosus with modified product specificity (2011), Arch. Biochem. Biophys., 505, 118-122.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| crtI gene, construction of different mutation libraries used for screening for mutants which synthesize almost exclusively either neurosporene or lycopene, cloning n Escherichia coli strain DH5alpha | Rubrivivax gelatinosus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D355G | naturally occuring mutation | Rubrivivax gelatinosus |
| D53G | naturally occuring mutation | Rubrivivax gelatinosus |
| L153P | naturally occuring mutation, which influences the secondary structure of the enzyme | Rubrivivax gelatinosus |
| L208F | naturally occuring mutation, which influences the secondary structure of the enzyme | Rubrivivax gelatinosus |
| L208P | naturally occuring mutation, which influences the secondary structure of the enzyme | Rubrivivax gelatinosus |
| L278P | naturally occuring mutation, which influences the secondary structure of the enzyme | Rubrivivax gelatinosus |
| L424P | naturally occuring mutation, which influences the secondary structure of the enzyme | Rubrivivax gelatinosus |
| additional information | in mutant EP21, zeta-carotene and phytofluene, which are both early intermediates of the desaturation pathway and which are absent in the wild-type, are the main carotene products | Rubrivivax gelatinosus |
| P134L | naturally occuring mutation, which influences the secondary structure of the enzyme | Rubrivivax gelatinosus |
| T256M | naturally occuring mutation | Rubrivivax gelatinosus |
| V395A | naturally occuring mutation | Rubrivivax gelatinosus |
| Y44C | naturally occuring mutation | Rubrivivax gelatinosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0048 | - |
phytoene | pH 8.0, 28°C, recombinant mutant L208F | Rubrivivax gelatinosus |  |
| 0.0148 | - |
phytoene | pH 8.0, 28°C, recombinant wild-type enzyme | Rubrivivax gelatinosus | |
| 0.0171 | - |
phytoene | pH 8.0, 28°C, recombinant mutant L208P | Rubrivivax gelatinosus | |
| 0.0331 | - |
7,9,9'-tricis-neurosporene | pH 8.0, 28°C, recombinant wild-type enzyme | Rubrivivax gelatinosus | |
| 0.0417 | - |
7,9,9'-tricis-neurosporene | pH 8.0, 28°C, recombinant mutant L208P | Rubrivivax gelatinosus | |
| 0.0658 | - |
7,9,9'-tricis-neurosporene | pH 8.0, 28°C, recombinant mutant L208F | Rubrivivax gelatinosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | transmembrane enzyme, model, overview | Rubrivivax gelatinosus | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,9,9'-tricis-neurosporene + a quinone | Rubrivivax gelatinosus | - |
7,9,7',9'-tetracis-lycopene + a quinol | - |
? | |
| 7,9,9'-tricis-neurosporene + a quinone | Rubrivivax gelatinosus CGMCC 6086 | - |
7,9,7',9'-tetracis-lycopene + a quinol | - |
? | |
| phytoene + quinone | Rubrivivax gelatinosus | - |
7,9,9'-tricis-neurosporene + quinol | - |
? | |
| phytoene + quinone | Rubrivivax gelatinosus CGMCC 6086 | - |
7,9,9'-tricis-neurosporene + quinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rubrivivax gelatinosus | - |
gene crtI | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,9,9'-tricis-neurosporene + a quinone | - |
Rubrivivax gelatinosus | 7,9,7',9'-tetracis-lycopene + a quinol | - |
? | |
| 7,9,9'-tricis-neurosporene + a quinone | - |
Rubrivivax gelatinosus CGMCC 6086 | 7,9,7',9'-tetracis-lycopene + a quinol | - |
? | |
| phytoene + quinone | - |
Rubrivivax gelatinosus | 7,9,9'-tricis-neurosporene + quinol | - |
? | |
| phytoene + quinone | - |
Rubrivivax gelatinosus CGMCC 6086 | 7,9,9'-tricis-neurosporene + quinol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CrtI | - |
Rubrivivax gelatinosus |
| phytoene desaturase | - |
Rubrivivax gelatinosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Rubrivivax gelatinosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Rubrivivax gelatinosus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | phytoene desaturase CrtI from Rubrivivax gelatinosus catalyzes simultaneously a three- and four-step desaturation producing both neurosporene and lycopene. These carotenes are intermediates for the synthesis of spheroidene and spirilloxanthin, respectively | Rubrivivax gelatinosus |
| additional information | Leu208, located in a highly hydrophobic membrane-integrated region, is important in substrate binding | Rubrivivax gelatinosus |
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