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Literature summary for 1.3.5.6 extracted from

  • Wang, C.W.; Liao, J.C.
    Alteration of product specificity of Rhodobacter sphaeroides phytoene desaturase by directed evolution (2001), J. Biol. Chem., 276, 41161-41164.
    View publication on PubMed
Search for more literature for 1.3.5.6

Protein Variants

Protein Variants Comment Organism
E508G random mutagenesis, the mutation has little effect on the enzyme activity Cereibacter sphaeroides
F166I random mutagenesis, a beneficial mutation that helps the conversion of product from neurosporene to lycopene Cereibacter sphaeroides
F220S random mutagenesis, the mutation has little effect on the enzyme activity Cereibacter sphaeroides
H12Q random mutagenesis, the mutation has little effect on the enzyme activity Cereibacter sphaeroides
L148H random mutagenesis, the beneficial mutation is highly increasing the production of lycopene to over 80% Cereibacter sphaeroides
M402T random mutagenesis, the mutation reduces the lycopene production rate Cereibacter sphaeroides
additional information alteration of product specificity of Rhodobacter sphaeroides phytoene desaturase by directed evolution combining random mutagenesis and site-directed mutagenesis, DNA sequence determinations. Beneficial mutations increase the percent of lycopene production to 90% Cereibacter sphaeroides
additional information introduction of stop mutations, M1, M2 and M3 Cereibacter sphaeroides
V68D random mutagenesis, a beneficial mutation that helps the conversion of product from neurosporene to lycopene Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phytoene + quinone Cereibacter sphaeroides via phytofluene and zeta-carotene 7,9,9'-tricis-neurosporene + quinol
-
 ?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
 gene crtI
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,9,9'-tricis-neurosporene + a quinone mutant activity Cereibacter sphaeroides 7,9,7',9'-tetracis-lycopene + a quinol
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 ?
phytoene + quinone via phytofluene and zeta-carotene Cereibacter sphaeroides 7,9,9'-tricis-neurosporene + quinol
-
 ?

Synonyms

Synonyms Comment Organism
phytoene desaturase
-
 Cereibacter sphaeroides

General Information

General Information Comment Organism
evolution approximately 10% of known phytoene desaturases, as in Rhodobacter, produce neurosporene, whereas the rest produce lycopene. These two types of enzymes, although similar in function, have relatively low similarity, below 60%, in terms of nucleotide or amino acid sequence. Phytoene desaturase encoded by gene crtI is a neurosporene-producing enzyme Cereibacter sphaeroides
physiological function phytoene desaturase encoded by crtI catalyzes the desaturation of phytoene in the carotenoid biosynthesis pathway, approximately 10% of known phytoene desaturases, as in Rhodobacter, produce neurosporene, whereas the rest produce lycopene, mechanism controlling the product specificity, phytoene desaturase encoded by gene crtI is a neurosporene-producing enzyme, overview Cereibacter sphaeroides