BRENDA - Enzyme Database show
show all sequences of 1.3.7.12

Molecular cloning, functional expression and characterisation of RCC reductase involved in chlorophyll catabolism

Wüthrich, K.L.; Bovet, L.; Hunziker, P.E.; Donnison, I.S.; Hörtensteiner, S.; Plant J. 21, 189-198 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
a partial gene sequence
Hordeum vulgare
expression in Escherichia coli
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
O2
reaction is sensitive to oxygen
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
AtRCCR import is inhibited when the protein is missing 40 amino acids at the N-terminus
Arabidopsis thaliana
9507
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
2 * 31000, SDS-PAGE
Hordeum vulgare
58000
-
gel filtration
Hordeum vulgare
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Hordeum vulgare
the major product of reduction of red chlorophyll catabolite is pFCC1, but small quantities of its C1 epimer, pFCC-2, also accumulate. Red chlorophyll catabolite reductase and pheophorbide a oxygenase catalyse the key eaction of chlorophyll catabolism, porphin macrocycle cleavage of pheide a to a primary fluorescent catabolite
?
-
-
-
additional information
Arabidopsis thaliana
the major product of reduction of red chlorophyll catabolite is pFCC1, but small quantities of its C1 epimer, pFCC-2, also accumulate. Red chlorophyll catabolite reductase and pheophorbide a oxygenase catalyse the key reaction of chlorophyll catabolism, porphin macrocycle cleavage of pheide a to a primary fluorescent catabolite
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Hordeum vulgare
Q9MTQ6
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
proteolytic modification
AtRCCR encodes a 35000 Da protein that is processed to a mature form of 31000 Da
Arabidopsis thaliana
Purification (Commentary)
Commentary
Organism
-
Arabidopsis thaliana
-
Hordeum vulgare
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
the gene is expressed at all stages of leaf development
Hordeum vulgare
-
leaf
senescent
Arabidopsis thaliana
-
root
-
Hordeum vulgare
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the major product of reduction of red chlorophyll catabolite is pFCC1, but small quantities of its C1 epimer, pFCC-2, also accumulate. Red chlorophyll catabolite reductase and pheophorbide a oxygenase catalyse the key eaction of chlorophyll catabolism, porphin macrocycle cleavage of pheide a to a primary fluorescent catabolite
676490
Hordeum vulgare
?
-
-
-
-
additional information
the major product of reduction of red chlorophyll catabolite is pFCC1, but small quantities of its C1 epimer, pFCC-2, also accumulate. Red chlorophyll catabolite reductase and pheophorbide a oxygenase catalyse the key reaction of chlorophyll catabolism, porphin macrocycle cleavage of pheide a to a primary fluorescent catabolite
676490
Arabidopsis thaliana
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 31000, SDS-PAGE
Hordeum vulgare
Cofactor
Cofactor
Commentary
Organism
Structure
Ferredoxin
the reaction requires reduced ferredoxin
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
a partial gene sequence
Hordeum vulgare
expression in Escherichia coli
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
Ferredoxin
the reaction requires reduced ferredoxin
Arabidopsis thaliana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
O2
reaction is sensitive to oxygen
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
AtRCCR import is inhibited when the protein is missing 40 amino acids at the N-terminus
Arabidopsis thaliana
9507
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
2 * 31000, SDS-PAGE
Hordeum vulgare
58000
-
gel filtration
Hordeum vulgare
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Hordeum vulgare
the major product of reduction of red chlorophyll catabolite is pFCC1, but small quantities of its C1 epimer, pFCC-2, also accumulate. Red chlorophyll catabolite reductase and pheophorbide a oxygenase catalyse the key eaction of chlorophyll catabolism, porphin macrocycle cleavage of pheide a to a primary fluorescent catabolite
?
-
-
-
additional information
Arabidopsis thaliana
the major product of reduction of red chlorophyll catabolite is pFCC1, but small quantities of its C1 epimer, pFCC-2, also accumulate. Red chlorophyll catabolite reductase and pheophorbide a oxygenase catalyse the key reaction of chlorophyll catabolism, porphin macrocycle cleavage of pheide a to a primary fluorescent catabolite
?
-
-
-
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
proteolytic modification
AtRCCR encodes a 35000 Da protein that is processed to a mature form of 31000 Da
Arabidopsis thaliana
Purification (Commentary) (protein specific)
Commentary
Organism
-
Arabidopsis thaliana
-
Hordeum vulgare
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
the gene is expressed at all stages of leaf development
Hordeum vulgare
-
leaf
senescent
Arabidopsis thaliana
-
root
-
Hordeum vulgare
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the major product of reduction of red chlorophyll catabolite is pFCC1, but small quantities of its C1 epimer, pFCC-2, also accumulate. Red chlorophyll catabolite reductase and pheophorbide a oxygenase catalyse the key eaction of chlorophyll catabolism, porphin macrocycle cleavage of pheide a to a primary fluorescent catabolite
676490
Hordeum vulgare
?
-
-
-
-
additional information
the major product of reduction of red chlorophyll catabolite is pFCC1, but small quantities of its C1 epimer, pFCC-2, also accumulate. Red chlorophyll catabolite reductase and pheophorbide a oxygenase catalyse the key reaction of chlorophyll catabolism, porphin macrocycle cleavage of pheide a to a primary fluorescent catabolite
676490
Arabidopsis thaliana
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 31000, SDS-PAGE
Hordeum vulgare
Other publictions for EC 1.3.7.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736190
Xiao
Cloning and expression analysi ...
Capsicum annuum
Genet. Mol. Res.
14
368-379
2015
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1
3
3
1
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726323
Liu
Nitric oxide deficiency accele ...
Arabidopsis thaliana
PLoS ONE
8
e56345
2013
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1
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726877
Sakuraba
7-Hydroxymethyl chlorophyll a ...
Arabidopsis thaliana
Biochem. Biophys. Res. Commun.
430
32-37
2013
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1
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726165
Sakuraba
STAY-GREEN and chlorophyll cat ...
Arabidopsis thaliana
Plant Cell
24
507-518
2012
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736688
Zhang
Correlation of leaf senescence ...
Brassica rapa
J. Plant Physiol.
168
2081-2087
2011
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2
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712769
Sugishima
Crystal structures of the subs ...
Arabidopsis thaliana
J. Mol. Biol.
402
879-891
2010
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3
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699585
Sugishima
Crystal structure of red chlor ...
Arabidopsis thaliana
J. Mol. Biol.
389
376-387
2009
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2
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3
3
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700695
Ougham
The control of chlorophyll cat ...
Arabidopsis thaliana
Plant Biol.
10 Suppl 1
4-14
2008
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2
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4
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676436
Pruzinska
In vivo participation of red c ...
Arabidopsis thaliana
Plant Cell
19
369-387
2007
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2
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671354
Hörtensteiner
Chlorophyll degradation during ...
Arabidopsis sp., Hordeum vulgare, Solanum lycopersicum, Spinacia oleracea
Annu. Rev. Plant Biol.
57
55-77
2006
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3
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3
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2
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3
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5
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1
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4
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1
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3
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2
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1
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4
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735438
Hoertensteiner
Chlorophyll degradation during ...
Arabidopsis thaliana
Annu. Plant Biol.
57
55-77
2006
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1
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1
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2
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2
1
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1
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2
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1
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1
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1
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1
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2
1
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3
3
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676587
Pruzinska
Chlorophyll breakdown in senes ...
Arabidopsis thaliana
Plant Physiol.
139
52-63
2005
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736960
Roca
Analysis of the chlorophyll ca ...
Lolium temulentum
Phytochemistry
65
1231-1238
2004
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2
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1
1
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676913
Mach
The Arabidopsis-accelerated ce ...
Arabidopsis sp.
Proc. Natl. Acad. Sci. USA
98
771-776
2001
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1
1
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676395
Hörtensteiner
-
Evolution of chlorophyll degra ...
Angiopteris, Auxenochlorella protothecoides, Carex, Cleome graveolens, Cycas sp., Equisetum sp., Euptelea, Ginkgo biloba, Hordeum vulgare, Metasequoia, Picea, Psilotum, Selaginella sp., Solanum lycopersicum, Spinacia oleracea, Taxus baccata, Taxus sp., Tropaeolum majus
Plant Biol.
2
63-67
2000
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2
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3
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27
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15
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18
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18
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38
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1
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1
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15
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15
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2
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3
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27
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15
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18
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38
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1
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1
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15
15
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676490
Wüthrich
Molecular cloning, functional ...
Arabidopsis thaliana, Hordeum vulgare
Plant J.
21
189-198
2000
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2
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1
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2
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5
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3
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1
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1
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1
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2
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3
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1
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736215
Muehlecker
-
Breakdown of chlorophyll: A fl ...
Brassica napus, Capsicum annuum
Helv. Chim. Acta
83
278-286
2000
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1
2
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2
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2
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2
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2
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2
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2
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3
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1
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8
8
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736949
Hoertensteiner
Chlorophyll breakdown in oilse ...
Arabidopsis thaliana, Brassica napus
Photosyn. Res.
64
137-146
2000
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1
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4
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2
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4
2
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4
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4
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2
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2
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1
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4
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2
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2
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4
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4
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4
4
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735913
Hoertensteiner
Chlorophyll breakdown in highe ...
Auxenochlorella protothecoides, Brassica napus, Capsicum annuum, Festuca pratensis, Hordeum vulgare, Parachlorella kessleri, Phaseolus vulgaris
Cell. Mol. Life Sci.
56
330-347
1999
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5
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6
14
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9
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9
5
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1
7
-
5
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16
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7
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7
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5
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6
14
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9
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5
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1
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5
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16
-
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-
17
17
-
-
-
674404
Hörtensteiner
The key step in chlorophyll br ...
Brassica napus
J. Biol. Chem.
273
15335-15339
1998
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2
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1
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5
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2
2
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676564
Rodoni
Chlorophyll breakdown in senes ...
Brassica napus
Plant Physiol.
115
669-676
1997
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1
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-
676565
Rodoni
Partial purification and chara ...
Hordeum vulgare
Plant Physiol.
115
677-682
1997
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1
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5
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