BRENDA - Enzyme Database show
show all sequences of 1.3.7.12

Cloning and expression analysis of pepper chlorophyll catabolite reductase gene CaRCCR

Xiao, H.J.; Jin, J.H.; Chai, W.G.; Gong, Z.H.; Genet. Mol. Res. 14, 368-379 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene CaRCCR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree
Capsicum annuum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Capsicum annuum
9507
-
additional information
the deduced CaRCCR protein contains a chloroplast transit peptide with 37-amino acid residues, its cleavage site was located between P37 and M38. The deduced CaRCCR does not have a signal peptide region or transmembrane helix
Capsicum annuum
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Capsicum annuum
V5K6J8
gene CaRCCR, cv. B12
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
immature
Capsicum annuum
-
leaf
highest enzyme expression level, the transcript level of CaRCCR is almost constant in the young leaves, fully expanded leaves, and senescent leaves
Capsicum annuum
-
additional information
constitutive expression during all phases of development, analyses of tissue-specific expression and various stress-induced expression patterns of gene CaRCCR via quantitative real-time PCR
Capsicum annuum
-
root
-
Capsicum annuum
-
stem
-
Capsicum annuum
-
Cofactor
Cofactor
Commentary
Organism
Structure
Ferredoxin
-
Capsicum annuum
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CaRCCR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree
Capsicum annuum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
Ferredoxin
-
Capsicum annuum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Capsicum annuum
9507
-
additional information
the deduced CaRCCR protein contains a chloroplast transit peptide with 37-amino acid residues, its cleavage site was located between P37 and M38. The deduced CaRCCR does not have a signal peptide region or transmembrane helix
Capsicum annuum
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
immature
Capsicum annuum
-
leaf
highest enzyme expression level, the transcript level of CaRCCR is almost constant in the young leaves, fully expanded leaves, and senescent leaves
Capsicum annuum
-
additional information
constitutive expression during all phases of development, analyses of tissue-specific expression and various stress-induced expression patterns of gene CaRCCR via quantitative real-time PCR
Capsicum annuum
-
root
-
Capsicum annuum
-
stem
-
Capsicum annuum
-
Expression
Organism
Commentary
Expression
Capsicum annuum
the enzyme is upregulated by abscisic acid, methyl jasmonate, and salicylic acid, and it is induced by high salinity and drought stress treatments. The enzyme expression is also slightly increased when the pepper plants are infected with Phytophthora capsici strain HX-9
up
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the ferredoxin-dependent bilin reductase family, FDBR, and contains two conserved acidic residue sites (Glu151 and Asp288), which are involved in catalysis and/or substrate binding
Capsicum annuum
metabolism
opening the porphyrin macrocycle of pheophorbide a and forming the primary fluorescent chlorophyll catabolites are key steps in the chlorophyll catabolism pathway. These steps are catalyzed by pheophorbide a oxygenase and red chlorophyll catabolite reductase
Capsicum annuum
physiological function
the enzyme is involved in defense responses to various stresses
Capsicum annuum
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the ferredoxin-dependent bilin reductase family, FDBR, and contains two conserved acidic residue sites (Glu151 and Asp288), which are involved in catalysis and/or substrate binding
Capsicum annuum
metabolism
opening the porphyrin macrocycle of pheophorbide a and forming the primary fluorescent chlorophyll catabolites are key steps in the chlorophyll catabolism pathway. These steps are catalyzed by pheophorbide a oxygenase and red chlorophyll catabolite reductase
Capsicum annuum
physiological function
the enzyme is involved in defense responses to various stresses
Capsicum annuum
Expression (protein specific)
Organism
Commentary
Expression
Capsicum annuum
the enzyme is upregulated by abscisic acid, methyl jasmonate, and salicylic acid, and it is induced by high salinity and drought stress treatments. The enzyme expression is also slightly increased when the pepper plants are infected with Phytophthora capsici strain HX-9
up
Other publictions for EC 1.3.7.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736190
Xiao
Cloning and expression analysi ...
Capsicum annuum
Genet. Mol. Res.
14
368-379
2015
-
-
1
-
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
1
3
3
1
-
-
726323
Liu
Nitric oxide deficiency accele ...
Arabidopsis thaliana
PLoS ONE
8
e56345
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
726877
Sakuraba
7-Hydroxymethyl chlorophyll a ...
Arabidopsis thaliana
Biochem. Biophys. Res. Commun.
430
32-37
2013
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
726165
Sakuraba
STAY-GREEN and chlorophyll cat ...
Arabidopsis thaliana
Plant Cell
24
507-518
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
736688
Zhang
Correlation of leaf senescence ...
Brassica rapa
J. Plant Physiol.
168
2081-2087
2011
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
1
1
2
-
-
712769
Sugishima
Crystal structures of the subs ...
Arabidopsis thaliana
J. Mol. Biol.
402
879-891
2010
-
-
1
1
2
-
-
-
1
-
-
2
-
2
-
-
1
-
-
1
-
-
4
2
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
1
2
-
-
-
-
-
1
-
-
2
-
-
-
1
-
1
-
-
4
2
-
-
-
-
-
-
-
-
-
3
3
-
-
-
699585
Sugishima
Crystal structure of red chlor ...
Arabidopsis thaliana
J. Mol. Biol.
389
376-387
2009
1
-
1
1
-
-
-
-
3
-
2
2
-
2
-
-
1
-
-
1
-
-
3
2
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
1
-
-
-
-
-
-
3
-
2
2
-
-
-
1
-
1
-
-
3
2
-
-
-
-
-
-
-
-
-
3
3
-
-
-
700695
Ougham
The control of chlorophyll cat ...
Arabidopsis thaliana
Plant Biol.
10 Suppl 1
4-14
2008
-
-
-
-
-
-
-
-
2
-
-
1
-
4
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676436
Pruzinska
In vivo participation of red c ...
Arabidopsis thaliana
Plant Cell
19
369-387
2007
-
-
-
-
2
-
-
-
1
-
-
1
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671354
Hörtensteiner
Chlorophyll degradation during ...
Arabidopsis sp., Hordeum vulgare, Solanum lycopersicum, Spinacia oleracea
Annu. Rev. Plant Biol.
57
55-77
2006
-
-
3
-
3
-
-
-
2
-
-
3
-
5
-
-
2
-
-
1
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
1
-
3
-
-
-
-
-
2
-
-
3
-
-
-
2
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
735438
Hoertensteiner
Chlorophyll degradation during ...
Arabidopsis thaliana
Annu. Plant Biol.
57
55-77
2006
-
-
1
-
1
-
-
-
2
-
2
1
-
1
-
-
-
-
-
2
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
2
-
2
1
-
-
-
-
-
2
-
-
2
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
676587
Pruzinska
Chlorophyll breakdown in senes ...
Arabidopsis thaliana
Plant Physiol.
139
52-63
2005
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736960
Roca
Analysis of the chlorophyll ca ...
Lolium temulentum
Phytochemistry
65
1231-1238
2004
-
-
-
-
1
-
-
-
2
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
676913
Mach
The Arabidopsis-accelerated ce ...
Arabidopsis sp.
Proc. Natl. Acad. Sci. USA
98
771-776
2001
-
1
1
-
-
-
-
-
2
-
-
1
-
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676395
Hörtensteiner
-
Evolution of chlorophyll degra ...
Angiopteris, Auxenochlorella protothecoides, Carex, Cleome graveolens, Cycas sp., Equisetum sp., Euptelea, Ginkgo biloba, Hordeum vulgare, Metasequoia, Picea, Psilotum, Selaginella sp., Solanum lycopersicum, Spinacia oleracea, Taxus baccata, Taxus sp., Tropaeolum majus
Plant Biol.
2
63-67
2000
-
-
-
-
2
-
3
-
27
-
-
15
-
18
-
-
-
-
-
18
-
-
38
-
1
-
-
-
1
-
-
15
-
-
-
-
-
-
15
-
2
-
-
3
-
-
27
-
-
15
-
-
-
-
-
18
-
-
38
-
1
-
-
-
1
-
-
-
-
15
15
-
-
-
676490
Wüthrich
Molecular cloning, functional ...
Arabidopsis thaliana, Hordeum vulgare
Plant J.
21
189-198
2000
-
-
2
-
-
-
1
-
1
-
2
2
-
5
-
1
2
-
-
3
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
2
1
-
-
-
-
1
-
-
1
-
2
2
-
-
1
2
-
3
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736215
Muehlecker
-
Breakdown of chlorophyll: A fl ...
Brassica napus, Capsicum annuum
Helv. Chim. Acta
83
278-286
2000
-
-
-
-
-
-
-
-
1
2
-
2
-
2
-
-
-
-
-
2
-
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
2
-
2
-
-
-
-
-
2
-
-
3
-
-
-
-
-
1
-
-
-
-
8
8
-
-
-
736949
Hoertensteiner
Chlorophyll breakdown in oilse ...
Arabidopsis thaliana, Brassica napus
Photosyn. Res.
64
137-146
2000
-
-
-
-
-
-
1
-
4
-
-
2
-
4
2
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
-
4
-
-
2
-
2
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
4
4
-
-
-
735913
Hoertensteiner
Chlorophyll breakdown in highe ...
Auxenochlorella protothecoides, Brassica napus, Capsicum annuum, Festuca pratensis, Hordeum vulgare, Parachlorella kessleri, Phaseolus vulgaris
Cell. Mol. Life Sci.
56
330-347
1999
-
-
-
-
-
-
5
-
6
14
-
9
-
9
5
-
1
7
-
5
-
-
16
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
7
-
-
-
-
5
-
-
6
14
-
9
-
5
-
1
-
5
-
-
16
-
-
-
-
-
-
-
-
-
-
17
17
-
-
-
674404
Hörtensteiner
The key step in chlorophyll br ...
Brassica napus
J. Biol. Chem.
273
15335-15339
1998
-
-
-
-
-
-
-
-
2
-
-
1
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
676564
Rodoni
Chlorophyll breakdown in senes ...
Brassica napus
Plant Physiol.
115
669-676
1997
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676565
Rodoni
Partial purification and chara ...
Hordeum vulgare
Plant Physiol.
115
677-682
1997
-
-
-
-
-
-
1
1
1
-
-
1
-
5
-
-
1
-
-
2
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
1
1
-
-
1
-
-
-
1
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-