Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of an enzyme BciB knockout mutant and recombinant expression of Rhodobacter sphaeroides gene bciA in the mutant | Synechocystis sp. PCC 6803 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Synechocystis sp. PCC 6803 | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+ | Synechocystis sp. PCC 6803 | preferred substrate | chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+ | - |
? | |
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+ | Synechocystis sp. PCC 6803 | is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide | protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+ | - |
? | |
additional information | Synechocystis sp. PCC 6803 | although the native BciB is able to reduce the 8V group of Pchlide, the preferred substrate for this enzyme is 8V Chlide, with only a small amount of 8E Pchlide detected in the wild-type when grown under pigment accumulating conditions | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. PCC 6803 | P74473 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+ | preferred substrate | Synechocystis sp. PCC 6803 | chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+ | - |
? | |
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+ | is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide | Synechocystis sp. PCC 6803 | protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+ | - |
? | |
additional information | although the native BciB is able to reduce the 8V group of Pchlide, the preferred substrate for this enzyme is 8V Chlide, with only a small amount of 8E Pchlide detected in the wild-type when grown under pigment accumulating conditions | Synechocystis sp. PCC 6803 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
8V reductase | - |
Synechocystis sp. PCC 6803 |
8VR | - |
Synechocystis sp. PCC 6803 |
bciB | - |
Synechocystis sp. PCC 6803 |
C8-vinyl reductase | - |
Synechocystis sp. PCC 6803 |
nucleoside-diphosphate-sugar epimerase | UniProt | Synechocystis sp. PCC 6803 |
RSP_3070 | - |
Synechocystis sp. PCC 6803 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ferredoxin [iron-sulfur] cluster | - |
Synechocystis sp. PCC 6803 | |
NADPH | - |
Synechocystis sp. PCC 6803 |
General Information | Comment | Organism |
---|---|---|
evolution | two unrelated classes of C8-vinyl reductase are known to exist, BciA and BciB | Synechocystis sp. PCC 6803 |
metabolism | the majority of (B)Chls utilized for light-harvesting carry an ethyl group at the C8 position (8E) of the macrocycle. This group is produced by the reduction of a vinyl group (8V), catalysed by an 8V reductase, 8VR, resulting in the production of an 8E pigment | Synechocystis sp. PCC 6803 |
additional information | BciB functions as an 8VR and does not require the presence of an additional subunit | Synechocystis sp. PCC 6803 |