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Literature summary for 1.3.7.15 extracted from

  • Nomata, J.; Mizoguchi, T.; Tamiaki, H.; Fujita, Y.
    A second nitrogenase-like enzyme for bacteriochlorophyll biosynthesis: reconstitution of chlorophyllide a reductase with purified X-protein (BchX) and YZ-protein (BchY-BchZ) from Rhodobacter capsulatus (2006), J. Biol. Chem., 281, 15021-15028.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
reconstitution of chlorophyllide a reductase with purified proteins. Two Rhodobacter capsulatus strains that overexpress Strep-tagged BchX and BchY are isolated. Strep-tagged BchX is purified as a single polypeptide, and BchZ is co-purified with Strep-tagged BchY. When BchX and BchY-BchZ components are incubated with chlorophyllide a, ATP, and dithionite under anaerobic conditions, chlorophyllide a is converted to a new pigment Rhodobacter capsulatus

Metals/Ions

Metals/Ions Comment Organism Structure
[4Fe-4S]-center
-
Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorophyllide a + AH2 + ATP + H2O Rhodobacter capsulatus
-
3-deacetyl-3-vinylbacteriochlorophyllide a + A + ADP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyllide a + AH2 + ATP + H2O
-
Rhodobacter capsulatus 3-deacetyl-3-vinylbacteriochlorophyllide a + A + ADP + phosphate
-
?
chlorophyllide a + AH2 + ATP + H2O the enzyme catalyses trans-reduction of the B-ring of chlorophyllide a. The product has the (7R,8R)-configuration Rhodobacter capsulatus 3-deacetyl-3-vinylbacteriochlorophyllide a + A + ADP + phosphate
-
?

Synonyms

Synonyms Comment Organism
BchX
-
Rhodobacter capsulatus
BchY
-
Rhodobacter capsulatus
BchZ
-
Rhodobacter capsulatus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
34
-
assay at Rhodobacter capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Rhodobacter capsulatus