Cloned (Comment) | Organism |
---|---|
reconstitution of chlorophyllide a reductase with purified proteins. Two Rhodobacter capsulatus strains that overexpress Strep-tagged BchX and BchY are isolated. Strep-tagged BchX is purified as a single polypeptide, and BchZ is co-purified with Strep-tagged BchY. When BchX and BchY-BchZ components are incubated with chlorophyllide a, ATP, and dithionite under anaerobic conditions, chlorophyllide a is converted to a new pigment | Rhodobacter capsulatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S]-center | - |
Rhodobacter capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyllide a + AH2 + ATP + H2O | Rhodobacter capsulatus | - |
3-deacetyl-3-vinylbacteriochlorophyllide a + A + ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyllide a + AH2 + ATP + H2O | - |
Rhodobacter capsulatus | 3-deacetyl-3-vinylbacteriochlorophyllide a + A + ADP + phosphate | - |
? | |
chlorophyllide a + AH2 + ATP + H2O | the enzyme catalyses trans-reduction of the B-ring of chlorophyllide a. The product has the (7R,8R)-configuration | Rhodobacter capsulatus | 3-deacetyl-3-vinylbacteriochlorophyllide a + A + ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BchX | - |
Rhodobacter capsulatus |
BchY | - |
Rhodobacter capsulatus |
BchZ | - |
Rhodobacter capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
34 | - |
assay at | Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rhodobacter capsulatus |