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Literature summary for 1.3.7.4 extracted from

  • Hu, L.; Liu, P.; Jin, Z.; Sun, J.; Weng, Y.; Chen, P.; Du, S.; Wei, A.; Li, Y.
    A mutation in CsHY2 encoding a phytochromobilin (P?B) synthase leads to an elongated hypocotyl 1(elh1) phenotype in cucumber (Cucumis sativus L.) (2021), Theor. Appl. Genet., 134, 2639-2652 .
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
gene CsHY2, genotyping, several segregating populations qre employed by crossing C1238 with the original parental line CCMC, the American pickling cucumber line Gy14 and the North China fresh marker type cucumber line 9930, respectively. For linkage mapping and cloning of the elh1 gene, F2 populations are derived from the C1238×9930 F1 and C1238×Gy14 F1 plants, respectively. Quantitative real-time PCR enzyme expression analysis. Phylogenetic analysis of CsHY2 homologues from different plants. Linkage mapping of the elh1 locus Cucumis sativus

Protein Variants

Protein Variants Comment Organism
additional information identification of elongated hypocotyl-1 (elh1) mutant C1238, comparison of mutant seedlings with wild-type CCMC seedlings, phenotype, overview. Analysis of the effect of CsHY2 mutation on the function of phys, the expression dynamics of cucumber PHYs genes (CsPHYA1, CsPHYA2, CsPHYB, CsPHYC, and CsPHYE). The 35S:CsHY2-EGFP plasmid construct is transformed into Agrobacterium tumefaciens strain GV3101. Arabidopsis hy2-1 recessive homozygous mutants are transformed and the phenotype is analyzed. The expression level of CsHY2 in mutant is the highest in male and female flowers, followed in order by stem, true leaves, root, cotyledon, and fruit. There is no significant difference in CsHY2 expression in all these organs except cotyledons, true leaves, and stem between wild-type and mutant elh1 Cucumis sativus

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast the CsHY2 protein sequence contains an N-terminal chloroplast transit peptide (cTP) Cucumis sativus 9507
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Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ in cofactor ferrdoxin Cucumis sativus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biliverdin IXalpha + 2 reduced ferredoxin Cucumis sativus
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 (3Z)-phytochromobilin + 2 oxidized ferredoxin
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 ?

Organism

Organism UniProt Comment Textmining
Cucumis sativus A0A0A0LXG3
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Source Tissue

Source Tissue Comment Organism Textmining
hypocotyl
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 Cucumis sativus
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additional information spatiotemporal expression patterns of CsHY2 Cucumis sativus
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seedling
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 Cucumis sativus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biliverdin IXalpha + 2 reduced ferredoxin
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 Cucumis sativus (3Z)-phytochromobilin + 2 oxidized ferredoxin
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 ?

Synonyms

Synonyms Comment Organism
Csa_1G616870
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 Cucumis sativus
CsHY2
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 Cucumis sativus
HY2 protein
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 Cucumis sativus
phytochromobilin synthase
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 Cucumis sativus
PphiB synthase
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 Cucumis sativus

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
 Cucumis sativus

General Information

General Information Comment Organism
evolution phylogenetic analysis of CsHY2 homologues from different plants Cucumis sativus
malfunction the long hypocotyl mutant C1238 is identified from an EMS-induced mutagenesis population of the cucumber inbred line CCMC with normal hypocotyl and green leaves. The mutant C1238 exhibits apparently elongated hypocotyl and yellow-green leaves at the seedling stage Cucumis sativus
physiological function the CsHY2 protein plays a role in the chloroplast, which is consistent with the plastid localization of PPhiB synthesis and the RNA-seq data, suggesting that tetrapyrrole chromophore biosynthesis and chlorophyll metabolism are mainly in the chloroplast in Cucumis sativus Cucumis sativus