Cloned (Comment) | Organism |
---|---|
Acaryochloris marina strain 11017 encodes two PcyA homologues, AmPcyAc and AmPcyAp. AmPcyAc is encoded on the main chromosome with most photoreceptor genes, whereas AmPcyAp is encoded on a plasmid with PBS-related genes, these PcyA homologues share 56% sequence identity. Cloning of gene pcyA or AM1_5311 and recombinant expression of the His-tagged enzyme in Escherichia coli strain C41, phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis | Acaryochloris marina |
Acaryochloris marina strain 11017 encodes two PcyA homologues, AmPcyAc and AmPcyAp. AmPcyAc is encoded on the main chromosome with most photoreceptor genes, whereas AmPcyAp is encoded on a plasmid with PBS-related genes, these PcyA homologues share 56% sequence identity. Cloning of gene pcyA or AM1_C0185 and recombinant expression of the His-tagged enzyme in Escherichia coli strain C41, phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis | Acaryochloris marina |
gene pcyA, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain C41, coexpression with heme oxygenase 1 (HO1) | Synechocystis sp. PCC 6803 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | in cofactor ferredoxin | Synechocystis sp. PCC 6803 | |
Fe2+ | in cofactor ferredoxin | Acaryochloris marina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin IXalpha + 4 reduced ferredoxin | Synechocystis sp. PCC 6803 | - |
(3Z)-phycocyanobilin + 4 oxidized ferredoxin | - |
? | |
biliverdin IXalpha + 4 reduced ferredoxin | Acaryochloris marina | - |
(3Z)-phycocyanobilin + 4 oxidized ferredoxin | - |
? | |
biliverdin IXalpha + 4 reduced ferredoxin | Acaryochloris marina MBIC 11017 | - |
(3Z)-phycocyanobilin + 4 oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acaryochloris marina | A8ZMS5 | gene pcyA or AM1_C0185 encoding enzyme AmPcyAp | - |
Acaryochloris marina | B0CAR8 | gene pcyA or AM1_5311 encoding enzyme AmPcyAc | - |
Acaryochloris marina MBIC 11017 | A8ZMS5 | gene pcyA or AM1_C0185 encoding enzyme AmPcyAp | - |
Acaryochloris marina MBIC 11017 | B0CAR8 | gene pcyA or AM1_5311 encoding enzyme AmPcyAc | - |
Synechocystis sp. PCC 6803 | Q55891 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain C41 by nickel affinity chromatography and dialysis | Synechocystis sp. PCC 6803 |
recombinant His-tagged enzyme from Escherichia coli strain C41 by nickel affinity chromatography and dialysis | Acaryochloris marina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin IXalpha + 4 reduced ferredoxin | - |
Synechocystis sp. PCC 6803 | (3Z)-phycocyanobilin + 4 oxidized ferredoxin | - |
? | |
biliverdin IXalpha + 4 reduced ferredoxin | - |
Acaryochloris marina | (3Z)-phycocyanobilin + 4 oxidized ferredoxin | - |
? | |
biliverdin IXalpha + 4 reduced ferredoxin | via the intermediate 181,182-dihydrobiliverdin (181/182-DHBV) | Acaryochloris marina | (3Z)-phycocyanobilin + 4 oxidized ferredoxin | - |
? | |
biliverdin IXalpha + 4 reduced ferredoxin | via the intermediate 181/182-dihydrobiliverdin (181/182-DHBV) | Acaryochloris marina | (3Z)-phycocyanobilin + 4 oxidized ferredoxin | - |
? | |
biliverdin IXalpha + 4 reduced ferredoxin | - |
Acaryochloris marina MBIC 11017 | (3Z)-phycocyanobilin + 4 oxidized ferredoxin | - |
? | |
biliverdin IXalpha + 4 reduced ferredoxin | via the intermediate 181,182-dihydrobiliverdin (181/182-DHBV) | Acaryochloris marina MBIC 11017 | (3Z)-phycocyanobilin + 4 oxidized ferredoxin | - |
? | |
biliverdin IXalpha + 4 reduced ferredoxin | via the intermediate 181/182-dihydrobiliverdin (181/182-DHBV) | Acaryochloris marina MBIC 11017 | (3Z)-phycocyanobilin + 4 oxidized ferredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30100, recombinant enzyme AmPcyAp, SDS-PAGE | Acaryochloris marina |
? | x * 30200, recombinant enzyme AmPcyAc, SDS-PAGE | Acaryochloris marina |
? | x * 30400, recombinant enzyme AmPcyAp, SDS-PAGE | Synechocystis sp. PCC 6803 |
Synonyms | Comment | Organism |
---|---|---|
AmPcyAc | - |
Acaryochloris marina |
AmPcyAp | - |
Acaryochloris marina |
HY2 protein | - |
Synechocystis sp. PCC 6803 |
HY2 protein | - |
Acaryochloris marina |
PcyA | - |
Synechocystis sp. PCC 6803 |
PcyA | - |
Acaryochloris marina |
phycocyanobilin:ferredoxin oxidoreductase | - |
Synechocystis sp. PCC 6803 |
phycocyanobilin:ferredoxin oxidoreductase | - |
Acaryochloris marina |
SyPcyA | - |
Synechocystis sp. PCC 6803 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Synechocystis sp. PCC 6803 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Synechocystis sp. PCC 6803 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Synechocystis sp. PCC 6803 | |
Ferredoxin | - |
Acaryochloris marina |
General Information | Comment | Organism |
---|---|---|
evolution | Acaryochloris marina strain 11017 evolved to utilize specific PcyA enzymes for sensing wide range of spectrum upon both PCB and 181/182-DHBV binding and harvesting short-wavelength orange light upon only PCB binding. Phycocyanin a from Acaryochloris marina strain 11017 binds only phycocyanobilin (PCB) but not 181/182-DHBV | Acaryochloris marina |
evolution | Acaryochloris marina strain 11017 evolved to utilize specific PcyA enzymes for sensing wide range of spectrum upon both PCB and 181/182-DHBV binding and harvesting shortwavelength orange light upon only PCB binding. Phycocyanin a from Acaryochloris marina strain 11017 binds only phycocyanobilin (PCB) but not 181/182-DHBV | Acaryochloris marina |
metabolism | Acaryochloris marina strain 11017 exceptionally encodes two PcyA homologues, AmPcyAc and AmPcyAp. The enzymes AmPcyAc and AmPcyAp show completely different kinetics especially for 181/182-DHBV accumulation. High accumulation of 181/182-DHBV for extended periods is observed during the reaction catalyzed by AmPcyAc, whereas 181/182-DHBV is transiently accumulated for a short period during the reaction catalyzed by AmPcyAp. Phycocyanin a from Acaryochloris marina strain 11017 binds only phycocyanobilin (PCB) but not 181/182-DHBV | Acaryochloris marina |
physiological function | phycocyanobilin:ferredoxin oxidoreductase (PcyA) catalyzes PCB synthesis from biliverdin via the intermediate 181/182-dihydrobiliverdin (181/182-DHBV), resulting in the stepwise shortening of the absorbing wavelengths. Cyanobacteriochromes (CBCRs) sense longer wavelength far-red light through 181/182-DHBV incorporation, whereas phycocyanobilin (PCB) only harvests orange light through PCB incorporation, suggesting functional diversification of PcyA as AmPcyAc and AmPcyAp to provide 181/182-DHBV and PCB to the light perception and harvesting systems, respectively. While most cyanobacteria utilize chlorophyll a as the photosynthetic reaction center pigment, Acaryochloris marina 11017 exceptionally utilizes chlorophyll d that absorbs longer wavelength far-red light than chlorophyll a | Acaryochloris marina |
physiological function | phycocyanobilin:ferredoxin oxidoreductase (PcyA) catalyzes PCB synthesis from biliverdin via the intermediate 181/182-dihydrobiliverdin (181/182-DHBV), resulting in the stepwise shortening of the absorbing wavelengths. Cyanobacteriochromes (CBCRs) sense longer wavelength far-red light through 181/182-DHBV incorporation, whereas phycocyanobilin (PCB) only harvests orange light through PCB incorporation, suggesting functional diversification of PcyA as AmPcyAc and AmPcyAp to provide 181/182-DHBV and PCB to the light perception and harvesting systems, respectively. While most cyanobacteria utilize chlorophyll a as the photosynthetic reaction center pigment, Acaryochloris marina strain 11017 exceptionally utilizes chlorophyll d that absorbs longer wavelength far-red light than chlorophyll a | Acaryochloris marina |