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Literature summary for 1.3.7.7 extracted from
- Reconstitution of light-independent protochlorophyllide reductase from purified bchl and BchN-BchB subunits. In vitro confirmation of nitrogenase-like features of a bacteriochlorophyll biosynthesis enzyme (2000), J. Biol. Chem., 275, 23583-23588.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Dithionite | dark protochlorophyllide reductase activity is dependent on the reductant dithionite | Rhodobacter capsulatus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| subunits BchL and BchN are expressed in Rhodobacter capsulatus strains YCN1 or YCL3 as S tag fusion proteins, subunit BchB is co-purified with the BchN protein | Rhodobacter capsulatus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
2 * 31000, L-protein of DPOR, SDS-PAGE | Rhodobacter capsulatus |
| 33413 | - |
2 * 33413, L-protein of DPOR, calculated from amino acid sequence | Rhodobacter capsulatus |
| 43000 | - |
2 * 51000 + 2 * 43000 , NB-protein of DPOR, SDS-PAGE | Rhodobacter capsulatus |
| 46038 | - |
2 * 57191 + 2 * 46038, NB-protein of DPOR, calculated from amino acid sequence | Rhodobacter capsulatus |
| 51000 | - |
2 * 51000 + 2 * 43000 , NB-protein of DPOR, SDS-PAGE | Rhodobacter capsulatus |
| 57191 | - |
2 * 57191 + 2 * 46038, NB-protein of DPOR, calculated from amino acid sequence | Rhodobacter capsulatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodobacter capsulatus | - |
strain strain CB1029 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| S protein-agarose column chromatography | Rhodobacter capsulatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protochlorophyllide + oxidized ferredoxin + ADP + phosphate | - |
Rhodobacter capsulatus | chlorophyllide a + reduced ferredoxin + ATP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | 2 * 51000 + 2 * 43000 , NB-protein of DPOR, SDS-PAGE | Rhodobacter capsulatus |
| heterotetramer | 2 * 57191 + 2 * 46038, NB-protein of DPOR, calculated from amino acid sequence | Rhodobacter capsulatus |
| homodimer | 2 * 31000, L-protein of DPOR, SDS-PAGE | Rhodobacter capsulatus |
| homodimer | 2 * 33413, L-protein of DPOR, calculated from amino acid sequence | Rhodobacter capsulatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dark protochlorophyllide reductase | - |
Rhodobacter capsulatus |
| DPOR | - |
Rhodobacter capsulatus |
| light-independent protochlorophyllide reductase | - |
Rhodobacter capsulatus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Rhodobacter capsulatus | |
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