Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyclohex-1-ene-1-carboxyl-CoA | monoenoyl-CoA | Geobacter metallireducens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | in iron-sulfur clusters | Geobacter metallireducens | |
Mg2+ | required | Geobacter metallireducens | |
additional information | the Bam(BC)2 heterotetramer contains iron-sulfur clusters, tungsten, and zinc | Geobacter metallireducens | |
Tungsten | - |
Geobacter metallireducens | |
Zinc | - |
Geobacter metallireducens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | Geobacter metallireducens | - |
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? | |
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | Geobacter metallireducens GS-15 | - |
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? | |
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | Geobacter metallireducens DSM 7210 | - |
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? | |
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | Geobacter metallireducens ATCC 53774 | - |
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacter metallireducens | - |
- |
- |
Geobacter metallireducens ATCC 53774 | - |
- |
- |
Geobacter metallireducens DSM 7210 | - |
- |
- |
Geobacter metallireducens GS-15 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate = benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | benzoyl-CoA reduction is initiated by a hydrogen atom transfer from a W(IV) species with an aqua ligand, yielding W(V)-[OH-] and a substrate radical intermediate. In the next step, a proton-assisted second electron transfer takes place with a conserved active-site histidine serving as the second proton donor. The electron for the second reduction step is taken from the pyranopterin cofactors rather than from the tungsten ion. The resulting cationic radical, which is distributed over both pyranopterins, is stabilized by conserved anionic amino acid residues, reaction mechanism, detailed overview | Geobacter metallireducens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | - |
Geobacter metallireducens | cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? | |
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | substrate binding structure analysis | Geobacter metallireducens | cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? | |
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | - |
Geobacter metallireducens GS-15 | cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? | |
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | substrate binding structure analysis | Geobacter metallireducens GS-15 | cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? | |
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | - |
Geobacter metallireducens DSM 7210 | cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? | |
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | substrate binding structure analysis | Geobacter metallireducens DSM 7210 | cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? | |
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | - |
Geobacter metallireducens ATCC 53774 | cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? | |
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | substrate binding structure analysis | Geobacter metallireducens ATCC 53774 | cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BamB | - |
Geobacter metallireducens |
class II benzoyl-CoA reductase complex | - |
Geobacter metallireducens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Geobacter metallireducens | |
Fe-S center | the Bam(BC)2 heterotetramer contains iron-sulfur clusters. The BamC subunits, which presumably connect the BamBC to the rest of the BamB-I complex, bind three [4Fe-4S] clusters each | Geobacter metallireducens | |
Ferredoxin | - |
Geobacter metallireducens |
General Information | Comment | Organism |
---|---|---|
metabolism | in benzoic acid metabolism (Bam), the anaerobic bacterium Geobacter metallireducens initiates production of a class II BCR complex, when grown on benzoate. The complex is the eight-subunit complex BamBCDEFGHI. This BamB-I complex drives the endergonic benzoyl-CoA reduction to dienoyl-CoA presumably by flavin-based electron bifurcation instead of coupling to ATP hydrolysis. The BamBC part with BamB harbors the active site | Geobacter metallireducens |
additional information | continuum electrostatic and QM/MM calculations are used to model benzoyl-CoA reduction by BamB and elucidate the reaction mechanism. The Bam(BC)2 heterotetramer contains iron-sulfur clusters, tungsten, and zinc, analysis of Bam(BC)2 heterotetramer structure with the redox cofactors ([4Fe-4S] clusters and bis-WPT) and the substrate benzoyl-CoA. The BamC subunits, which presumably connect the BamBC to the rest of the BamB-I complex, bind three [4Fe-4S] clusters each. Substrate binding structure in the active site of the BamBC dimer, overview | Geobacter metallireducens |
physiological function | in anaerobic microorganisms, most monocyclic aromatic growth substrates are converted to the central intermediate benzoyl-coenzyme A, which is enzymatically reduced to cyclohexa-1,5-dienoyl-CoA. The strictly anaerobic bacterium Geobacter metallireducens uses the class II benzoyl-CoA reductase complex for this reaction. The catalytic BamB subunit of this complex harbors an active site tungsten-bispyranopterin cofactor with the metal being coordinated by five protein/cofactor-derived sulfur atoms and a sixth ligand | Geobacter metallireducens |