Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, wild-type and Glu367Gln mutant enzyme | Megasphaera elsdenii |
Protein Variants | Comment | Organism |
---|---|---|
E367Q | mutant enzyme shows less than 0.03% of the activity seen in normal cloned enzyme, E367 is responsible for catalytic activity | Megasphaera elsdenii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Megasphaera elsdenii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and E367Q mutant enzyme | Megasphaera elsdenii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
0.1-0.4 mM/min/micromol of FAD, enzyme activity in crude extracts and activity of purified cloned enzyme | Megasphaera elsdenii |