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Literature summary for 1.3.8.1 extracted from

  • Battaile, K.P.; Mohsen, A.W.A.; Vockley, J.
    Functional role of the active site glutamate-368 in rat short chain Acyl-CoA dehydrogenase (1996), Biochemistry, 35, 15356-15363.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type, E368G/G247E, E368D, E368Q and E368G mutant enzyme in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
E368D mutant exhibits enzyme activity Rattus norvegicus
E368G inactive enzyme, E368 is responsible for catalytic activity Rattus norvegicus
E368G/G247E mutant exhibits enzyme activity Rattus norvegicus
E368L inactive enzyme, E368 is responsible for catalytic activity Rattus norvegicus
E368Q inactive enzyme, E368 is responsible for catalytic activity Rattus norvegicus
E368R inactive enzyme, E368 is responsible for catalytic activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
butyryl-CoA wild-type enzyme Rattus norvegicus
0.0011
-
octanoyl-CoA E368G/G247E double mutant enzyme Rattus norvegicus
0.0015
-
butyryl-CoA E368D mutant enzyme Rattus norvegicus
0.0017
-
hexanoyl-CoA E368G/G247E double mutant enzyme Rattus norvegicus
0.002
-
butyryl-CoA E368G/G247E double mutant enzyme Rattus norvegicus
0.0038
-
hexanoyl-CoA wild-type enzyme Rattus norvegicus
0.005
-
octanoyl-CoA E368D mutant enzyme Rattus norvegicus
0.005
-
hexanoyl-CoA E368D mutant enzyme Rattus norvegicus
0.0081
-
octanoyl-CoA wild-type enzyme Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type enzyme purified, E368Q, E368G and E368L mutant enzyme, partially purified Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
E368G/G247E double mutant enzyme, electron transfer protein as electron acceptor Rattus norvegicus
1.9
-
E368D mutant enzyme, electron transfer protein as electron acceptor Rattus norvegicus
3.8
-
wild-type enzyme, electron transfer protein as electron acceptor Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
octanoyl-CoA + electron transfer protein
-
Rattus norvegicus 2-octenoyl-CoA + reduced acceptor
-
?