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Literature summary for 1.3.8.1 extracted from

  • Sedlmeier, H.; Simon, H.
    Purification and some properties of an acryloyl-CoA reductase of Clostridium kluyveri (1985), Biol. Chem. Hoppe-Seyler, 366, 953-961.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
diethyl dicarbonate 1 mM, 83% residual activity Clostridium kluyveri
iodoacetamide 1 mM, 38% residual activity Clostridium kluyveri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00083
-
acrylyl-CoA 22°C, pH 6.4 Clostridium kluyveri
0.008
-
(E)-2-butenoyl-CoA 22°C, pH 6.4 Clostridium kluyveri
0.035
-
N-acetyl-S-acryloyl-cysteamine 22°C, pH 6.4 Clostridium kluyveri
0.77
-
ethyl vinyl ketone 22°C, pH 6.4 Clostridium kluyveri
5.6
-
acrolein 22°C, pH 6.4 Clostridium kluyveri

Metals/Ions

Metals/Ions Comment Organism Structure
Iron less than 0.2 g-atom per mol protein Clostridium kluyveri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14200
-
2 * 14200, SDS-PAGE Clostridium kluyveri
28400
-
gel filtration Clostridium kluyveri

Organism

Organism UniProt Comment Textmining
Clostridium kluyveri
-
-
-

Oxidation Stability

Oxidation Stability Organism
enzyme is not oxygen-sensitive Clostridium kluyveri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
170
-
22°C, pH 6.4 Clostridium kluyveri

Storage Stability

Storage Stability Organism
-18°C, 30% ethylene glycol, 15 mM phosphate pH 8.0, 20% loss of activity within 1 year Clostridium kluyveri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-2-butenoyl-CoA + reduced methylviologen
-
Clostridium kluyveri ? + oxidized methylviologen
-
?
acrolein + reduced methylviologen
-
Clostridium kluyveri ? + oxidized methylviologen
-
?
acryloyl-CoA + reduced methylviologen
-
Clostridium kluyveri propanoyl-CoA + oxidized methylviologen
-
?
ethyl vinyl ketone + reduced methylviologen
-
Clostridium kluyveri ? + oxidized methylviologen
-
?
N-acetyl-S-acryloyl-cysteamine + reduced methylviologen
-
Clostridium kluyveri ? + oxidized methylviologen
-
?

Subunits

Subunits Comment Organism
dimer 2 * 14200, SDS-PAGE Clostridium kluyveri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.9
-
and 6.4 Clostridium kluyveri
6.4
-
and 5.9 Clostridium kluyveri

pH Range

pH Minimum pH Maximum Comment Organism
3.9
-
30% resiudal activity Clostridium kluyveri
8.3
-
30% residual activity Clostridium kluyveri

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
enzyme is more stable at pH 8.0 than at pH 6.0 Clostridium kluyveri

Cofactor

Cofactor Comment Organism Structure
FMN about 1.5 per mol of dimer Clostridium kluyveri

pI Value

Organism Comment pI Value Maximum pI Value
Clostridium kluyveri polyacrylamide slab gel
-
6.9
Clostridium kluyveri chromatofocusing
-
7.5