Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl dicarbonate | 1 mM, 83% residual activity | Clostridium kluyveri | |
iodoacetamide | 1 mM, 38% residual activity | Clostridium kluyveri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00083 | - |
acrylyl-CoA | 22°C, pH 6.4 | Clostridium kluyveri | |
0.008 | - |
(E)-2-butenoyl-CoA | 22°C, pH 6.4 | Clostridium kluyveri | |
0.035 | - |
N-acetyl-S-acryloyl-cysteamine | 22°C, pH 6.4 | Clostridium kluyveri | |
0.77 | - |
ethyl vinyl ketone | 22°C, pH 6.4 | Clostridium kluyveri | |
5.6 | - |
acrolein | 22°C, pH 6.4 | Clostridium kluyveri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | less than 0.2 g-atom per mol protein | Clostridium kluyveri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14200 | - |
2 * 14200, SDS-PAGE | Clostridium kluyveri |
28400 | - |
gel filtration | Clostridium kluyveri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium kluyveri | - |
- |
- |
Oxidation Stability | Organism |
---|---|
enzyme is not oxygen-sensitive | Clostridium kluyveri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
170 | - |
22°C, pH 6.4 | Clostridium kluyveri |
Storage Stability | Organism |
---|---|
-18°C, 30% ethylene glycol, 15 mM phosphate pH 8.0, 20% loss of activity within 1 year | Clostridium kluyveri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-2-butenoyl-CoA + reduced methylviologen | - |
Clostridium kluyveri | ? + oxidized methylviologen | - |
? | |
acrolein + reduced methylviologen | - |
Clostridium kluyveri | ? + oxidized methylviologen | - |
? | |
acryloyl-CoA + reduced methylviologen | - |
Clostridium kluyveri | propanoyl-CoA + oxidized methylviologen | - |
? | |
ethyl vinyl ketone + reduced methylviologen | - |
Clostridium kluyveri | ? + oxidized methylviologen | - |
? | |
N-acetyl-S-acryloyl-cysteamine + reduced methylviologen | - |
Clostridium kluyveri | ? + oxidized methylviologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 14200, SDS-PAGE | Clostridium kluyveri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | - |
and 6.4 | Clostridium kluyveri |
6.4 | - |
and 5.9 | Clostridium kluyveri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.9 | - |
30% resiudal activity | Clostridium kluyveri |
8.3 | - |
30% residual activity | Clostridium kluyveri |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme is more stable at pH 8.0 than at pH 6.0 | Clostridium kluyveri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | about 1.5 per mol of dimer | Clostridium kluyveri |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Clostridium kluyveri | polyacrylamide slab gel | - |
6.9 |
Clostridium kluyveri | chromatofocusing | - |
7.5 |