Literature summary for 1.3.8.10 extracted from

Kung, J.; Seifert, J.; von Bergen, M.; Bolla, M.
Cyclohexanecarboxyl-coenzyme a (CoA) and cyclohex-1-ene-1-carboxyl-CoA dehydrogenases, two enzymes involved in the fermentation of benzoate and crotonate in Syntrophus aciditrophicus (2013), J. Bacteriol., 195, 3193-3200.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL 21 Star(DE3) OneShot cells	Syntrophus aciditrophicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.005	-	cyclohex-1-ene-1-carbonyl-CoA	Km less than 0.005 mM, at pH 7.0 and 30°C	Syntrophus aciditrophicus
0.019	-	cyclohex-1,5-diene-1-carbonyl-CoA	at pH 7.0 and 30°C	Syntrophus aciditrophicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
150000	-	gel filtration	Syntrophus aciditrophicus

Organism

Organism	UniProt	Comment	Textmining
Syntrophus aciditrophicus	-	-	-
Syntrophus aciditrophicus SB	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-chelating affinity column chromatography	Syntrophus aciditrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.1	-	using (E)-2-cyclohex-1,5-diene-1-carbonyl-CoA as substrate, at pH 7.0 and 30°C	Syntrophus aciditrophicus
9.5	-	using cyclohex-1-ene-1-carbonyl-CoA as substrate, at pH 7.0 and 30°C	Syntrophus aciditrophicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cyclohex-1,5-diene-1-carbonyl-CoA + Ti(III) citrate	Ti(III) citrate (5mM) in the presence of methyl viologen (1 mM) serves as the electron donor	Syntrophus aciditrophicus	cyclohex-1-ene-1-carbonyl-CoA + oxidized Ti(III) citrate	-	r
cyclohex-1,5-diene-1-carbonyl-CoA + Ti(III) citrate	Ti(III) citrate (5mM) in the presence of methyl viologen (1 mM) serves as the electron donor	Syntrophus aciditrophicus SB	cyclohex-1-ene-1-carbonyl-CoA + oxidized Ti(III) citrate	-	r
cyclohex-1-ene-1-carbonyl-CoA + ferricenium hexafluorophosphate	-	Syntrophus aciditrophicus	cyclohex-1,5-diene-1-carbonyl-CoA + ferrocenium hexafluorophosphate	-	r
cyclohex-1-ene-1-carbonyl-CoA + ferricenium hexafluorophosphate	-	Syntrophus aciditrophicus SB	cyclohex-1,5-diene-1-carbonyl-CoA + ferrocenium hexafluorophosphate	-	r
cyclohexa-1,5-diene-1-carbonyl-CoA + ferricenium hexafluorophosphate	-	Syntrophus aciditrophicus	benzoyl-CoA + ferrocenium hexafluorophosphate	-	ir
cyclohexa-1,5-diene-1-carbonyl-CoA + ferricenium hexafluorophosphate	-	Syntrophus aciditrophicus SB	benzoyl-CoA + ferrocenium hexafluorophosphate	-	ir
additional information	no activity with ChCoA, 6-hydroxycyclohex-1-en-1-carboxyl-CoA, butyryl-CoA, glutaryl-CoA, succinyl-CoA, or cyclohexane carboxylate	Syntrophus aciditrophicus	?	-	?
additional information	no activity with ChCoA, 6-hydroxycyclohex-1-en-1-carboxyl-CoA, butyryl-CoA, glutaryl-CoA, succinyl-CoA, or cyclohexane carboxylate	Syntrophus aciditrophicus SB	?	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 40000-45000, SDS-PAGE	Syntrophus aciditrophicus

Synonyms

Synonyms	Comment	Organism
Ch1CoA dehydrogenase	-	Syntrophus aciditrophicus
cyclohex-1-ene-1-carboxyl-CoA dehydrogenase	-	Syntrophus aciditrophicus
cyclohexanecarboxyl-CoA dehydrogenase	-	Syntrophus aciditrophicus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Syntrophus aciditrophicus

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Release 2023.1 (January 2023)