Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 6.6 mM, 48% inhibition | Escherichia coli | |
choline | 200 mM, about 50% residual activity | Escherichia coli | |
Cu2+ | 1 mM, 100% inhibition | Escherichia coli | |
D-carnitine | - |
Escherichia coli | |
dithioerythritol | 6.6 mM, 85% inhibition | Escherichia coli | |
EDTA | 3.3 mM, 32% inhibition | Escherichia coli | |
Fe2+ | 1 mM, 94% inhibition | Escherichia coli | |
gamma-butyrobetaine | competitive, 100 mM, complete loss of activity | Escherichia coli | |
glutathione | 6.6 mM, 67% inhibition | Escherichia coli | |
Hg2+ | 1 mM, 97% inhibition | Escherichia coli | |
Tris-HCl | - |
Escherichia coli | |
Zn2+ | 1 mM, 58% inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.7 | - |
crotonobetaine | pH 8.0, 37°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P60584 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonobetaine + reduced benzylviologen | - |
Escherichia coli | gamma-butyrobetaine + oxidized benzylviologen | - |
? | |
gamma-butyrobetainyl-CoA + electron-transfer flavoprotein | - |
Escherichia coli | crotonobetainyl-CoA + reduced electron-transfer flavoprotein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
caiA | - |
Escherichia coli |
crotonobetaine reductase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
Tris-HCl buffer is inhibitory | Escherichia coli |
7.8 | - |
potassium phosphate buffer | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
gamma-butyrobetaine | pH 8.0, 37°C | Escherichia coli |
Organism | Comment | Expression |
---|---|---|
Escherichia coli | inducible enzyme detectable only in cells grown anaerobically in the presence of L-carnitine or crotonobetaine as inducers | additional information |
Escherichia coli | fumarate causes a stimulation of growth and an increased expression of crotonobetaine reductase | up |