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Literature summary for 1.3.8.16 extracted from

  • Van Lanen, S.G.; Lin, S.; Shen, B.
    Biosynthesis of the enediyne antitumor antibiotic C-1027 involves a new branching point in chorismate metabolism (2008), Proc. Natl. Acad. Sci. USA, 105, 494-499.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.8.16

Cloned(Commentary)

Cloned (Comment) Organism
cloned into pET-30 Xa/LIC and expressed in Escherichia coli Streptomyces globisporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 FMN
-
 Streptomyces globisporus
0.056
-
 2-amino-2-deoxyisochorismate
-
 Streptomyces globisporus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron-sulfur enzyme. SgcG contains a [Fe-S] cluster with all four Cys found in SgcG involved in iron coordination Streptomyces globisporus
Mg2+ activity depends on Streptomyces globisporus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29100
-
 x * 29100, SDS-PAGE Streptomyces globisporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-amino-2-deoxyisochorismate + FMN Streptomyces globisporus the sequential action of 2-amino-4-deoxychorismate synthase (EC 2.6.1.86) and EC 1.3.99.24 leads to the formation of the benzoxazolinate moiety of the enediyne antitumour antibiotic C-1027 3-enolpyruvoylanthranilate + FMNH2
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces globisporus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Streptomyces globisporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-2-deoxyisochorismate + FMN
-
 Streptomyces globisporus 3-enolpyruvoylanthranilate + FMNH2
-
 ?
2-amino-2-deoxyisochorismate + FMN the sequential action of 2-amino-4-deoxychorismate synthase (EC 2.6.1.86) and EC 1.3.99.24 leads to the formation of the benzoxazolinate moiety of the enediyne antitumour antibiotic C-1027 Streptomyces globisporus 3-enolpyruvoylanthranilate + FMNH2
-
 ?

Subunits

Subunits Comment Organism
? x * 29100, SDS-PAGE Streptomyces globisporus

Synonyms

Synonyms Comment Organism
ADIC dehydrogenase
-
 Streptomyces globisporus
SgcG
-
 Streptomyces globisporus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15
-
 2-amino-2-deoxyisochorismate
-
 Streptomyces globisporus
17
-
 FMN
-
 Streptomyces globisporus

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Streptomyces globisporus