Literature summary for 1.3.8.4 extracted from

Nasser, I.; Mohsen, A.W.; Jelesarov, I.; Vockley, J.; Macheroux, P.; Ghisla, S.
Thermal unfolding of medium-chain acyl-CoA dehydrogenase and iso(3)valeryl-CoA dehydrogenase: study of the effect of genetic defects on enzyme stability (2004), Biochim. Biophys. Acta, 1690, 22-32.

Search for more literature for 1.3.8.4

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant enzyme in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
A282V	site-directed mutagenesis, severely affected interaction between enzyme and flavin cofactor, about 40% reduced activity compared to the wild-type enzyme	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
2-aza-isovaleryl-CoA	substrate analogue	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
isovaleryl-CoA + electron transfer protein	Homo sapiens	-	3-methylcrotonyl-CoA + reduced electron transfer protein	-	?
additional information	Homo sapiens	physiological implications of enzyme mutations and deficiency	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
isovaleryl-CoA + electron transfer protein	-	Homo sapiens	3-methylcrotonyl-CoA + reduced electron transfer protein	-	?
additional information	physiological implications of enzyme mutations and deficiency	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
i3VD	-	Homo sapiens
iso(3)valeryl-CoA dehydrogenase	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	thermal unfolding analysis	Homo sapiens
48.2	-	melting point of mutant A282V without bound ligand	Homo sapiens
48.5	-	melting point of mutant A282V with isovaleryl-CoA bound	Homo sapiens
48.6	-	melting point of mutant A282V with 2-aza-isovaleryl-CoA bound	Homo sapiens
50.5	-	melting point of wild-type enzyme without bound ligand	Homo sapiens
50.9	-	melting point of wild-type enzyme with 2-aza-isovaleryl-CoA bound	Homo sapiens
55.4	-	melting point of wild-type enzyme with isovaleryl-CoA bound	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
flavin	-	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)