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Literature summary for 1.3.8.7 extracted from

  • Ikeda, Y.; Okamura-Ikeda, K.; Tanaka, K.
    Purification and characterization of short-chain, medium-chain, and long-chain acyl-CoA dehydrogenases from rat liver mitochondria. Isolation of the holo- and apoenzymes and conversion of the apoenzyme to the holoenzyme (1985), J. Biol. Chem., 260, 1311-1325.
    View publication on PubMed
Search for more literature for 1.3.8.7

Inhibitors

Inhibitors Comment Organism Structure
2-hexadecenoyl-CoA competitive, long-chain acyl-CoA dehdrogenase Rattus norvegicus
additional information not inhibited by saturated and 2,3-unsaturated branched chain acyl-CoA compounds Rattus norvegicus
octenoyl-CoA competitive, medium-chain acyl-CoA dehydrogenase Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 octanoyl-CoA
-
 Rattus norvegicus
0.0045
-
 electron transfer flavoprotein
-
 Rattus norvegicus
0.0054
-
 decanoyl-CoA
-
 Rattus norvegicus
0.0057
-
 dodecanoyl-CoA
-
 Rattus norvegicus
0.0094
-
 hexanoyl-CoA
-
 Rattus norvegicus
0.0396
-
 pentanoyl-CoA
-
 Rattus norvegicus
0.135
-
 butanoyl-CoA
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 4 * 45000, SDS-PAGE Rattus norvegicus
180000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.96
-
 long-chain acyl-CoA dehydrogenase Rattus norvegicus
10 10.2
-
 Rattus norvegicus
11.9
-
 medium-chain acyl-CoA dehydrogenase Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + acceptor electron acceptor: phenazine methosulfate Rattus norvegicus 2,3-dehydroacyl-CoA + reduced acceptor
-
 ?
acyl-CoA + acceptor intermediary electron acceptor: 2,6-dichloroindophenol Rattus norvegicus 2,3-dehydroacyl-CoA + reduced acceptor
-
 ?
acyl-CoA + acceptor final electron acceptor: 8-dimethylamino-2,3-benzophenoxazinium chloride, trivial name meldola's blue Rattus norvegicus 2,3-dehydroacyl-CoA + reduced acceptor
-
 ?
butanoyl-CoA + acceptor
-
 Rattus norvegicus crotonyl-CoA + reduced acceptor
-
 ?
dodecanoyl-CoA + acceptor
-
 Rattus norvegicus 2-dodecenoyl-CoA + reduced acceptor
-
 ?
n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol best substrate for medium-chain acyl-CoA dehydrogenase Rattus norvegicus 2-octenoyl-CoA + reduced acceptor
-
 ?
n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol no activity with tetradecanoyl-CoA and hexadecanoyl-CoA Rattus norvegicus 2-octenoyl-CoA + reduced acceptor
-
 ?
n-tetradecanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol best substrate for long-chain acyl-CoA dehydrogenase Rattus norvegicus 2-tetradecenoyl-CoA + reduced acceptor
-
 ?
pentanoyl-CoA + acceptor
-
 Rattus norvegicus 2-pentenoyl-CoA + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 45000, SDS-PAGE Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6
-
 tetradecanoyl-CoA long-chain acyl-CoA dehydrogenase Rattus norvegicus
31.2
-
 hexanoyl-CoA medium-chain acyl-CoA dehydrogenase Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.7
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD 1 mol per mol of subunit Rattus norvegicus