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Literature summary for 1.3.8.7 extracted from
- Acyl-CoA dehydrogenase from pig kidney (1981), Methods Enzymol., 71, 366-374.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| dialysis against distilled water at 4°C | Sus scrofa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyldicarbonate | rapid inactivation | Sus scrofa |  |
| iodoacetic acid | S-carboxymethylated enzyme binds octanoyl-CoA but can no longer be reduced by it | Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
4 * 42000, SDS-PAGE | Sus scrofa |
| 160000 | - |
gel filtration | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 100 mM phosphate buffer, pH 7.6 | Sus scrofa |
| 4°C, ammonium sulfate suspension | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + acceptor | electron acceptor: phenazine methosulfate | Sus scrofa | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
| acyl-CoA + acceptor | intermediary electron acceptor: 2,6-dichloroindophenol | Sus scrofa | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
| acyl-CoA + acceptor | final electron acceptor: 8-dimethylamino-2,3-benzophenoxazinium chloride, trivial name meldola's blue | Sus scrofa | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 42000, SDS-PAGE | Sus scrofa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.25 | - |
octanoyl-CoA | phenazine methosulfate and 2,6-dichlorophenolindophenol as electron acceptors | Sus scrofa | |
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Release 2023.1 (January 2023)
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