Application | Comment | Organism |
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medicine | use of yeast enzyme as a positive selectable marker for transfections of Plasmodium falciparum, including its use in gene disruption strategies. A transfection vector designed for gene disruption, containing the yeast DHODH gene as the positive selection marker in combination with a previously described fused yeast cytosine deaminase-uracil phosphoribosyl transferase gene as a negative selection marker, yields positively selected parasites containing the plasmid after transfection of the Plasmodium falciparum D10 strain followed by selection with atovaquone. Yeast DHODH transgenic parasites can be selected in strains D10 and Dd2 by Plasmodium DHODH-specific triazolopyrimidine-based inhibitors | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
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Saccharomyces cerevisiae | - |
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