Cloned (Comment) | Organism |
---|---|
gene hemW or hemN, recombinant expression in the hemN-deficient Escherichia coli mutant strain JW3838 and in strain BL21(DE3) as His-tagged protein. Lactococcus lactis HemW at high levels does not complement the growth deficit of the Escherichia coli DELTAhemN mutant | Lactococcus lactis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | in vivo, HemW occurs as a heme-free cytosolic form, as well as a heme-containing membrane-associated form | Lactococcus lactis | 5829 | - |
membrane | in vivo, HemW occurs as a heme-free cytosolic form, as well as a heme-containing membrane-associated form | Lactococcus lactis | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a heme containing enzyme, HemN contains a [4Fe-4S] cluster | Lactococcus lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Lactococcus lactis | HemW shows no coproporphyrinogen III oxidase activity in vivo or in vitro | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
gene hemW or hemN | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged HemN from Escherichia coli strain BL21(DE3) membranes by nickel affinity chromatography and gel filtration | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | HemW shows no coproporphyrinogen III oxidase activity in vivo or in vitro | Lactococcus lactis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HemW | - |
Lactococcus lactis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | in vivo, HemW occurs as a heme-free cytosolic form, as well as a heme-containing membrane-associated form | Lactococcus lactis | |
S-adenosyl-L-methionine | - |
Lactococcus lactis |
General Information | Comment | Organism |
---|---|---|
evolution | HemW-like proteins form a distinct phylogenetic clade. It contains the four cysteine residues of the radical S-adenosyl-L-methionine enzyme motif of CPDH enzymes, structure comparisons, overview. The fourth cysteine residue of the Fe-S cluster motif of Escherichia coli HemN, CX3CX2CXC, is replaced by phenylalanine in HemW and related proteins | Lactococcus lactis |
metabolism | genes hemH and hemW (hemN) show conjectured functions in heme metabolism | Lactococcus lactis |
physiological function | addition of Lactococcus lactis membranes to heme-containing HemW triggers the release of heme from HemW in vitro. Role of HemW in heme trafficking | Lactococcus lactis |