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Literature summary for 1.3.99.17 extracted from

  • Bauder, R.; Tshisuaka, B.; Lingens, F.
    VII. Quinoline oxidoreductase from Pseudomonas putida a molybdenum-containing enzyme (1990), Biol. Chem. Hoppe-Seyler, 371, 1137-1144.
    View publication on PubMed
Search for more literature for 1.3.99.17

Activating Compound

Activating Compound Comment Organism Structure
ethanol 1 M, increases activity by about 10% Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
CN-
-
 Pseudomonas putida
methanol 0.7 M, 50% inhibition Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.182
-
 Quinoline with 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride as cosubstrate Pseudomonas putida
1.08
-
 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride with quinoline as cosubstrate Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe contains 8 atoms of iron and 8 atoms of acid-labile sulfide Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 x * 20000 + x * 30000 + x * 85000, SDS-PAGE Pseudomonas putida
30000
-
 x * 20000 + x * 30000 + x * 85000, SDS-PAGE Pseudomonas putida
85000
-
 x * 20000 + x * 30000 + x * 85000, SDS-PAGE Pseudomonas putida
300000
-
 gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida 86
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.2
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-chloroquinoline + p-iodonitrotetrazolium violet
-
 Pseudomonas putida ?
-
 ?
5-hydroxyquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida ?
-
 ?
5-hydroxyquinoline + acceptor acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue Pseudomonas putida ?
-
 ?
5-hydroxyquinoline + acceptor 15% of the activity with quinoline Pseudomonas putida ?
-
 ?
5-hydroxyquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida 86 ?
-
 ?
5-hydroxyquinoline + acceptor acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue Pseudomonas putida 86 ?
-
 ?
6-hydroxyquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida ?
-
 ?
6-hydroxyquinoline + acceptor acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue Pseudomonas putida ?
-
 ?
6-hydroxyquinoline + acceptor 19% of the activity with quinoline Pseudomonas putida ?
-
 ?
7-hydroxyquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida ?
-
 ?
7-hydroxyquinoline + acceptor acceptor: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue Pseudomonas putida ?
-
 ?
8-chloroquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida ?
-
 ?
8-chloroquinoline + acceptor acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue Pseudomonas putida ?
-
 ?
8-hydroxyquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida ?
-
 ?
8-hydroxyquinoline + acceptor acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue Pseudomonas putida ?
-
 ?
quinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida 2-oxo-1,2-dihydroquinoline + reduced acceptor
-
 ?
quinoline + acceptor acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride] Pseudomonas putida 2-oxo-1,2-dihydroquinoline + reduced acceptor
-
 ?
quinoline + acceptor 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue Pseudomonas putida 2-oxo-1,2-dihydroquinoline + reduced acceptor
-
 ?
quinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida 86 2-oxo-1,2-dihydroquinoline + reduced acceptor
-
 ?
quinoline + acceptor acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride] Pseudomonas putida 86 2-oxo-1,2-dihydroquinoline + reduced acceptor
-
 ?
quinoline + acceptor 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue Pseudomonas putida 86 2-oxo-1,2-dihydroquinoline + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
? x * 20000 + x * 30000 + x * 85000, SDS-PAGE Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD enzyme contains 2 moleculs of FAD Pseudomonas putida
molybdopterin
-
 Pseudomonas putida
additional information NAD+ and NADP+ are ineffective as cofactors Pseudomonas putida