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Literature summary for 1.3.99.19 extracted from

  • Bauer, G.; Lingens, F.
    Microbial metabolism of quinoline and related compounds. XV. Quinoline-4-carboxylic acid oxidoreductase from Agrobacterium spec. 1B: a molybdenum-containing enzyme (1992), Biol. Chem. Hoppe-Seyler, 373, 699-705.
    View publication on PubMed
Search for more literature for 1.3.99.19

Activating Compound

Activating Compound Comment Organism Structure
molybdopterin cytosine dinucleotide cofactor Agrobacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate
-
 Agrobacterium sp.
4-Chloroquinoline
-
 Agrobacterium sp.
4-Methylquinoline
-
 Agrobacterium sp.
Acriflavine
-
 Agrobacterium sp.
CN-
-
 Agrobacterium sp.
methanol
-
 Agrobacterium sp.
Quinoline
-
 Agrobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00008
-
 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride with quinoline-4-carboxylate as cosubstrate Agrobacterium sp.
0.0093
-
 Quinoline-4-carboxylate with 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride as cosubstrate Agrobacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Iron enzyme contains 8.3 g-atoms of iron and 7.2 mol of acid-labile sulfur per mol of enzyme Agrobacterium sp.
Molybdenum enzyme contains 2.1 mol of molybdenum per mol of enzyme Agrobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 or hexamer, 1 * or 2 * 21000 + 1 * or 2 * 35000 + 1 * or 2 * 85000, SDS-PAGE Agrobacterium sp.
35000
-
 or hexamer, 1 * or 2 * 21000 + 1 * or 2 * 35000 + 1 * or 2 * 85000, SDS-PAGE Agrobacterium sp.
85000
-
 or hexamer, 1 * or 2 * 21000 + 1 * or 2 * 35000 + 1 * or 2 * 85000, SDS-PAGE Agrobacterium sp.
320000
-
 gel filtration Agrobacterium sp.

Organism

Organism UniProt Comment Textmining
Agrobacterium sp.
-
 1B
-
Agrobacterium sp. 1B
-
 1B
-

Purification (Commentary)

Purification (Comment) Organism
-
 Agrobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.3
-
-
 Agrobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-chloroquinoline + acceptor with 4% of the activity relative to quinoline-4-carboxylate Agrobacterium sp. ?
-
 ?
4-chloroquinoline + acceptor with 4% of the activity relative to quinoline-4-carboxylate Agrobacterium sp. 1B ?
-
 ?
4-methylquinoline + acceptor with 24% of the activity relative to quinoline-4-carboxylate Agrobacterium sp. ?
-
 ?
4-methylquinoline + acceptor with 24% of the activity relative to quinoline-4-carboxylate Agrobacterium sp. 1B ?
-
 ?
quinoline + acceptor with 34% of the activity relative to quinoline-4-carboxylate Agrobacterium sp. ?
-
 ?
quinoline + acceptor with 34% of the activity relative to quinoline-4-carboxylate Agrobacterium sp. 1B ?
-
 ?
quinoline-4-carboxylate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O
-
 Agrobacterium sp. 2-oxo-1,2-dihydroquinoline-4-carboxylate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
-
 r
quinoline-4-carboxylate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O
-
 Agrobacterium sp. 1B 2-oxo-1,2-dihydroquinoline-4-carboxylate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
-
 r
quinoline-4-carboxylate + acceptor acceptors: 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride, thionin, menadione, benzoquinone, 2,6-dichlorophenol-indophenol Agrobacterium sp. 2-oxo-1,2-dihydroquinoline-4-carboxylic acid + reduced acceptor
-
 ?
quinoline-4-carboxylate + acceptor acceptors: 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride, thionin, menadione, benzoquinone, 2,6-dichlorophenol-indophenol Agrobacterium sp. 1B 2-oxo-1,2-dihydroquinoline-4-carboxylic acid + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
trimer or hexamer, 1 * or 2 * 21000 + 1 * or 2 * 35000 + 1 * or 2 * 85000, SDS-PAGE Agrobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Agrobacterium sp.

Cofactor

Cofactor Comment Organism Structure
FAD enzyme contains 1.7 mol of FAD per mol of enzyme Agrobacterium sp.