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Literature summary for 1.3.99.28 extracted from

  • Chi, S.C.; Mothersole, D.J.; Dilbeck, P.; Niedzwiedzki, D.M.; Zhang, H.; Qian, P.; Vasilev, C.; Grayson, K.J.; Jackson, P.J.; Martin, E.C.; Li, Y.; Holten, D.; Neil Hunter, C.
    Assembly of functional photosystem complexes in Rhodobacter sphaeroides incorporating carotenoids from the spirilloxanthin pathway (2015), Biochim. Biophys. Acta, 1847, 189-201 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis swapping the native three-step CrtI for the four-step Pantoea agglomerans enzyme, EC 1.3.99.31, reroutes carotenoid biosynthesis and culminates in the production of 2,2'-diketo-spirilloxanthin under semi-aerobic conditions. Premature termination of this pathway by inactivating crtC or crtD produces strains with lycopene or rhodopin as major carotenoids. All of the spirilloxanthin series carotenoids are accepted by the assembly pathways for light-harvesting 2 complex and reaction centre-light-harvesting 1-PufX complexes Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides P54980
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Cereibacter sphaeroides DSM 158 P54980
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Synonyms

Synonyms Comment Organism
CrtI
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Cereibacter sphaeroides