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Literature summary for 1.3.99.29 extracted from

  • Tran, P.; Sharifi, M.; Poddar, S.; Dent, R.; Niyogi, K.
    Intragenic enhancers and suppressors of phytoene desaturase mutations in Chlamydomonas reinhardtii (2012), PLoS ONE, 7, e42196.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.99.29

Cloned(Commentary)

Cloned (Comment) Organism
gene pds, DNA and amino acid sequence determination and analysis, sequence comarisons Chlamydomonas reinhardtii

Protein Variants

Protein Variants Comment Organism
E143K naturally occcuring mutation, mutation pds1-1 Chlamydomonas reinhardtii
L64F/E143K site-directed mutagenesis, mutation pds1-5, with in-frame deletion of PSY residues 7-14 Chlamydomonas reinhardtii
L64P site-directed mutagenesis, mutation pds1-4, with in-frame deletion of PSY residues 7-14 Chlamydomonas reinhardtii
L64P/E143K site-directed mutagenesis, mutation pds1-2, with in-frame deletion of PSY residues 7-14 Chlamydomonas reinhardtii
L64P/K90M/E143K site-directed mutagenesis, mutation pds1-6, with in-frame deletion of PSY residues 7-14 Chlamydomonas reinhardtii
additional information the naturally occuring pds1-1 allele shows a mutant with reduced enzyme activity and growth defects, isolation of pds1-2 as an intragenic enhancer of pds1-1, the pds1-2 and pds1-3 allele products completely lack PDS activity, pds1-3 is a null allele derived from DNA insertional mutagenesis. The null mutants accumulate only phytoene and no other carotenoids. All three phytoene-accumulating mutants exhibit lower growth rates and reduced plating efficiency compared to wild-type cells and white phytoene synthase mutants. DNA insertional mutagenesis, phenotypes, detailed overview Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
norflurazon a bleaching herbicide that specifically inhibits PDS activity and carotenoid biosynthesis Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
all-trans-phytofluene + acceptor Chlamydomonas reinhardtii
-
 all-trans-zeta-carotene + reduced acceptor
-
 ?
all-trans-phytofluene + acceptor Chlamydomonas reinhardtii 4A+
-
 all-trans-zeta-carotene + reduced acceptor
-
 ?
additional information Chlamydomonas reinhardtii the enzyme also forms 7,9,9'-tricis-neurosporene from 9,9'-dicis-zeta-carotene ?
-
 ?
additional information Chlamydomonas reinhardtii 4A+ the enzyme also forms 7,9,9'-tricis-neurosporene from 9,9'-dicis-zeta-carotene ?
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii Q6J213 gene pds
-
Chlamydomonas reinhardtii 4A+ Q6J213 gene pds
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans-phytofluene + acceptor
-
 Chlamydomonas reinhardtii all-trans-zeta-carotene + reduced acceptor
-
 ?
all-trans-phytofluene + acceptor
-
 Chlamydomonas reinhardtii 4A+ all-trans-zeta-carotene + reduced acceptor
-
 ?
additional information the enzyme also forms 7,9,9'-tricis-neurosporene from 9,9'-dicis-zeta-carotene Chlamydomonas reinhardtii ?
-
 ?
additional information the enzyme also forms 7,9,9'-tricis-neurosporene from 9,9'-dicis-zeta-carotene Chlamydomonas reinhardtii 4A+ ?
-
 ?

Synonyms

Synonyms Comment Organism
PDS
-
 Chlamydomonas reinhardtii
phytoene desaturase
-
 Chlamydomonas reinhardtii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Chlamydomonas reinhardtii

General Information

General Information Comment Organism
malfunction the light-sensitive, phytoene-accumulating mutant, pds1-1, isolated in Chlamydomonas reinhardtii, is leaky for PDS activity. It shows decreased accumulation of downstream colored carotenoids. The pds1-2 and pds1-3 allele products completely lack PDS activity, the null mutants accumulate only phytoene and no other carotenoids. All three phytoene-accumulating mutants exhibit lower growth rates and reduced plating efficiency compared to wild-type cells and white phytoene synthase mutants Chlamydomonas reinhardtii
metabolism the enzyme catalyzes the second step in carotenoid biosynthesis, the conversion of phytoene to zeta-carotene Chlamydomonas reinhardtii
additional information intragenic suppressors binding analysis and computational structure prediction of PDS, overview Chlamydomonas reinhardtii