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Literature summary for 1.3.99.30 extracted from

  • Hausmann, A.; Sandmann, G.
    A single five-step desaturase is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene in Neurospora crassa (2000), Fungal Genet. Biol., 30, 147-153.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NAD+ more than 2fold stimulation of activity compared to control, assay with supernatant of Escherichia coli recombinantly expressing phytoene desaturase from Neurospora crassa Neurospora crassa
NADP+ stimulation is lower than with NAD+, assay with supernatant of Escherichia coli recombinantly expressing phytoene desaturase from Neurospora crassa Neurospora crassa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
x * 62000, SDS-PAGE Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
15-cis-phytoene + 5 acceptor Neurospora crassa the enzyme is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Reaction

Reaction Comment Organism Reaction ID
15-cis-phytoene + 5 acceptor = all-trans-3,4-didehydrolycopene + 5 reduced acceptor overall reaction Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hydroxylycopene + acceptor
-
Neurospora crassa 1-hydroxy-3',4'-didehydrolycopene + reduced acceptor
-
?
1-hydroxyneurosporene + acceptor
-
Neurospora crassa 1-hydroxylycopene + reduced acceptor
-
?
15-cis-phytoene + 5 acceptor
-
Neurospora crassa all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
?
15-cis-phytoene + 5 acceptor the enzyme is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene Neurospora crassa all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
?
all-trans-lycopene + acceptor
-
Neurospora crassa all-trans-3,4-didehydrolycopene + reduced acceptor
-
?
all-trans-neurosporene + 2 acceptor
-
Neurospora crassa all-trans-3,4-didehydrolycopene + 2 reduced acceptor
-
?
all-trans-phytofluene + 4 acceptor
-
Neurospora crassa all-trans-3,4-didehydrolycopene + 4 reduced acceptor
-
?
all-trans-zeta-carotene + 3 acceptor
-
Neurospora crassa all-trans-3,4-didehydrolycopene + 3 reduced acceptor
-
?
additional information gamma-carotene and 1,1'-dihydroxylycopene are not accepted as substrate Neurospora crassa ?
-
?

Subunits

Subunits Comment Organism
? x * 62000, SDS-PAGE Neurospora crassa

Synonyms

Synonyms Comment Organism
Al-1
-
Neurospora crassa

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
assay at Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
additional information addition of FAD results in product formation close to that of the control without additions Neurospora crassa