BRENDA - Enzyme Database
show all sequences of 1.3.99.33

Urocanate reductase: identification of a novel anaerobic respiratory pathway in Shewanella oneidensis MR-1

Bogachev, A.V.; Bertsova, Y.V.; Bloch, D.A.; Verkhovsky, M.I.; Mol. Microbiol. 86, 1452-1463 (2012) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli
Shewanella oneidensis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
Urocanate
Km less than 0.01 mM, at pH 7.0 and 25°C
Shewanella oneidensis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Shewanella oneidensis
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
x * 60000, His-tagged enzyme, SDS-PAGE
Shewanella oneidensis
60200
-
x * 60200, His-tagged enzyme, calculated from amino acid sequence
Shewanella oneidensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
urocanate + reduced methyl viologen + H+
Shewanella oneidensis
-
dihydrourocanate + methyl viologen
-
-
?
urocanate + reduced methyl viologen + H+
Shewanella oneidensis MR-1 / ATCC 700550
-
dihydrourocanate + methyl viologen
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Shewanella oneidensis
Q8CVD0
-
-
Shewanella oneidensis MR-1 / ATCC 700550
Q8CVD0
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni-NTA column chromatography, and gel filtration
Shewanella oneidensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
the enzyme is unable to reduce cinnamate and shows no activity with acrylic, cinnamic, crotonic, fumaric and orotic acids
723218
Shewanella oneidensis
?
-
-
-
?
additional information
the enzyme is unable to reduce cinnamate and shows no activity with acrylic, cinnamic, crotonic, fumaric and orotic acids
723218
Shewanella oneidensis MR-1 / ATCC 700550
?
-
-
-
?
urocanate + reduced methyl viologen + H+
-
723218
Shewanella oneidensis
dihydrourocanate + methyl viologen
-
-
-
?
urocanate + reduced methyl viologen + H+
-
723218
Shewanella oneidensis MR-1 / ATCC 700550
dihydrourocanate + methyl viologen
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 60000, His-tagged enzyme, SDS-PAGE
Shewanella oneidensis
?
x * 60200, His-tagged enzyme, calculated from amino acid sequence
Shewanella oneidensis
Synonyms
Synonyms
Commentary
Organism
urdA
-
Shewanella oneidensis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
360
-
Urocanate
at pH 7.0 and 25°C
Shewanella oneidensis
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Shewanella oneidensis
FMN
-
Shewanella oneidensis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Shewanella oneidensis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Shewanella oneidensis
FMN
-
Shewanella oneidensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
Urocanate
Km less than 0.01 mM, at pH 7.0 and 25°C
Shewanella oneidensis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Shewanella oneidensis
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
x * 60000, His-tagged enzyme, SDS-PAGE
Shewanella oneidensis
60200
-
x * 60200, His-tagged enzyme, calculated from amino acid sequence
Shewanella oneidensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
urocanate + reduced methyl viologen + H+
Shewanella oneidensis
-
dihydrourocanate + methyl viologen
-
-
?
urocanate + reduced methyl viologen + H+
Shewanella oneidensis MR-1 / ATCC 700550
-
dihydrourocanate + methyl viologen
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography, and gel filtration
Shewanella oneidensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
the enzyme is unable to reduce cinnamate and shows no activity with acrylic, cinnamic, crotonic, fumaric and orotic acids
723218
Shewanella oneidensis
?
-
-
-
?
additional information
the enzyme is unable to reduce cinnamate and shows no activity with acrylic, cinnamic, crotonic, fumaric and orotic acids
723218
Shewanella oneidensis MR-1 / ATCC 700550
?
-
-
-
?
urocanate + reduced methyl viologen + H+
-
723218
Shewanella oneidensis
dihydrourocanate + methyl viologen
-
-
-
?
urocanate + reduced methyl viologen + H+
-
723218
Shewanella oneidensis MR-1 / ATCC 700550
dihydrourocanate + methyl viologen
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 60000, His-tagged enzyme, SDS-PAGE
Shewanella oneidensis
?
x * 60200, His-tagged enzyme, calculated from amino acid sequence
Shewanella oneidensis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
360
-
Urocanate
at pH 7.0 and 25°C
Shewanella oneidensis
Other publictions for EC 1.3.99.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723218
Bogachev
Urocanate reductase: identific ...
Shewanella oneidensis, Shewanella oneidensis MR-1 / ATCC 700550
Mol. Microbiol.
86
1452-1463
2012
-
-
1
-
-
-
-
1
1
-
2
2
-
12
-
-
1
-
-
-
-
-
4
2
1
-
-
-
1
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
1
1
-
2
2
-
-
-
1
-
-
-
-
4
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-