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Literature summary for 1.3.99.37 extracted from

  • Steiger, S.; Astier, C.; Sandmann, G.
    Substrate specificity of the expressed carotenoid 3,4-desaturase from Rubrivivax gelatinosus reveals the detailed reaction sequence to spheroidene and spirilloxanthin (2000), Biochem. J., 349, 635-640.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.99.37

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rubrivivax gelatinosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 1,1'-dihydroxyneurosporene pH 7.8, 28°C Rubrivivax gelatinosus
0.025
-
 1-hydroxy-neurosporene pH 7.8, 28°C Rubrivivax gelatinosus
0.04
-
 1-hydroxylycopene pH 7.8, 28°C Rubrivivax gelatinosus

Organism

Organism UniProt Comment Textmining
Rubrivivax gelatinosus Q9JPB5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,1'-dihydroxy-3,4-didehydrolycopene + acceptor
-
 Rubrivivax gelatinosus 1,1'-dihydroxy-3,4,3',4'-tetradehydrolycopene + reduced acceptor 38% conversion ?
1,1'-dihydroxyneurosporene + acceptor
-
 Rubrivivax gelatinosus 1'-hydroxydemethylspheroidene + reduced acceptor 39% conversion ?
1-hydroxy-3',4'-didehydrolycopene + acceptor
-
 Rubrivivax gelatinosus 1'-hydroxy-3,4,3',4'-tetradehydrolycopene + reduced acceptor 28% conversion ?
1-hydroxy-neurosporene + acceptor
-
 Rubrivivax gelatinosus demethylspheroidene + reduced acceptor 47% conversion ?
1-hydroxylycopene + acceptor
-
 Rubrivivax gelatinosus 1-hydroxy-3,4-didehydrolycopene + reduced acceptor 33% conversion ?
1-methoxy-1'-hydroxy-3,4-didehydrolycopene + acceptor
-
 Rubrivivax gelatinosus 1-methoxy-1'-hydroxy-3,4,3',4'-tetradehydrolycopene + reduced acceptor 37% conversion r
additional information The 3,4-desaturation reaction can only occur with mono-1-hydroxy carotenoids at a psi-end group or with 1,1'-dihydroxy derivatives carrying a 3',4'-double bond. 1-OH-zeta-carotene can also be converted by the desaturase. 1-OH-neurosporene is preferred over 1-OH-lycopene. No substrates are 1'-hydroxydemethylspheroidene, 1-methoxyneurosporene, 1,1'-dihydroxylycopene Rubrivivax gelatinosus ?
-
 ?

Synonyms

Synonyms Comment Organism
hydroxyneurosporene desaturase
-
 Rubrivivax gelatinosus