BRENDA - Enzyme Database show
show all sequences of 1.3.99.4

Purification and characterization of 3-ketosteroid-delta 1-dehydrogenase from Nocardia corallina

Itagaki, E.; Wakabayashi, T.; Hatta, T.; Biochim. Biophys. Acta 1038, 60-67 (1990)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1-Androstene-3,17-dione
-
Gordonia rubripertincta
estrone
-
Gordonia rubripertincta
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0063
-
11beta-hydroxytestosterone
i.e. 4-androstene-11beta,17beta-diol-3-one
Gordonia rubripertincta
0.0064
-
11-deoxy-17alpha-hydroxycorticosterone
i.e. 4-pregnene-17alpha,21-diol-3,20-dione
Gordonia rubripertincta
0.0066
-
androsterone
i.e. 4-androstene-3,11,17-trione
Gordonia rubripertincta
0.0083
-
19-nortestosterone
4-estren-17beta-ol-3one
Gordonia rubripertincta
0.0089
-
19-nor-4-androstene-3,17-dione
i.e. 4-estren-3,17-dione
Gordonia rubripertincta
0.0143
-
testololactone
i.e. 17alpha-oxo-D-homo-1,4-androstadiene-3,17-dione
Gordonia rubripertincta
0.0196
-
11alpha-hydroxyprogesterone
i.e. 4-pregnene-11alpha-ol-3,20-dione, 11beta-hydroxy-4-androstene-3,17-dione, 4-estren-3,17-dione
Gordonia rubripertincta
0.0204
-
corticosterone
i.e. 4-pregnene-11beta,21-diol-3,20-dione
Gordonia rubripertincta
0.021
-
4-androstene-3,17-dione
-
Gordonia rubripertincta
0.0256
-
cortisone
i.e. 4-pregnene-17alpha,21diol-3,11,20-trione
Gordonia rubripertincta
0.0385
-
hydrocorticosterone
i.e. 4-pregnene-11beta,17alpha,21-triol-3,20-dione
Gordonia rubripertincta
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60500
-
SDS-PAGE, gel filtration
Gordonia rubripertincta
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gordonia rubripertincta
-
inducible
-
Purification (Commentary)
Commentary
Organism
-
Gordonia rubripertincta
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
113
-
-
Gordonia rubripertincta
Storage Stability
Storage Stability
Organism
4°C, 1 week, stable
Gordonia rubripertincta
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11-deoxy-17alpha-hydroxycorticosterone + acceptor
4-pregnene-17alpha,21-diol-3,20-dione
391375
Gordonia rubripertincta
17,21-dihydroxypregna-1,4-diene-3,20-dione + reduced acceptor
-
-
-
?
11alpha-hydroxyprogesterone + acceptor
4-pregnene-11alpha-ol-3,20-dione
391375
Gordonia rubripertincta
11alpha-hydroxy-1,4-pregnene-3,20-dione + reduced acceptor
-
-
-
?
11beta-hydroxy-4-androstene-3,17-dione + acceptor
-
391375
Gordonia rubripertincta
11beta-hydroxy-1,4-androstene-3,17-dione + reduced acceptor
-
-
-
?
11beta-hydroxytestosterone + acceptor
4-androstene-11beta,17beta-diol-3-one
391375
Gordonia rubripertincta
11beta,17beta-dihydroxyandrost-1,4-diene-3-one + reduced acceptor
-
-
-
?
19-nor-4-androstene-3,17-dione + acceptor
4-estren-3,17-dione
391375
Gordonia rubripertincta
19-nor-1,4-androstene-3,17-dione + reduced acceptor
-
-
-
?
19-nortestosterone + acceptor
4-estren-17beta-ol-3-one
391375
Gordonia rubripertincta
17beta-hydroxy-1,4-estrene-3-one + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
-
391375
Gordonia rubripertincta
1,4-androstene-3,17-dione + reduced acceptor
-
-
-
?
androsterone + acceptor
4-androstene-3,11,17-trione
391375
Gordonia rubripertincta
3-alpha-hydroxy-1-androstene-17-one + reduced acceptor
-
-
-
?
corticosterone + acceptor
4-pregnene-11beta,21-diol-3,20-dione
391375
Gordonia rubripertincta
1,4-pregnene-17alpha,21-diol-3,20-dione + reduced acceptor
-
-
-
?
cortisone + acceptor
4-pregnene-17alpha,21-diol-3,11,20-trione
391375
Gordonia rubripertincta
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor
-
-
-
?
hydrocorticosterone + acceptor
4-pregnene-11beta,17alpha,21triol-3,20-dione
391375
Gordonia rubripertincta
1,4-pregnene-11beta,17alpha,21-triol-3,20-dione + reduced acceptor
-
-
-
?
additional information
acceptor: phenazine methosulfate
391375
Gordonia rubripertincta
?
-
-
-
-
additional information
acceptor: O2
391375
Gordonia rubripertincta
?
-
-
-
-
additional information
not: 3beta-hydroxysteroid, no electron acceptor: NADP+, K3Fe(CN)6, cytochrome c, 3-ketosteroids with a 11alpha- or 11beta-hydroxyl group are oxidized at slow rate, catalyzes aromatization of the A-ring of 19-nortestosterone and 19-norandrostenedione to produce estradiol and estrone, 19-hydroxytestosterone, 19-hydroxyandrosterone, 19-hydroxyandrostenedione and 19-oxotestosterone are converted to the respective phenolic steroids with cleavage of the C10 side-chain
391375
Gordonia rubripertincta
?
-
-
-
-
testololactone + acceptor
D-homo-17a-oxoandrost-4-ene-3,17-dione
391375
Gordonia rubripertincta
D-homo-17alpha-oxoandrosta-1,4-diene-3,17-one + reduced acceptor
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 60500, SDS-PAGE
Gordonia rubripertincta
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
activity increases linearly up to 45°C
Gordonia rubripertincta
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
20 min
Gordonia rubripertincta
52
-
20 min, 75% loss of activity
Gordonia rubripertincta
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
10
oxidase reaction
Gordonia rubripertincta
10
-
dehydrogenation, phenazine methosulfate
Gordonia rubripertincta
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
10.5
pH 7: about 60% of activity maximum, pH 10.5: about 85% of activity maximum, phenazine methosulfate dehydrogenation
Gordonia rubripertincta
8
11
pH 8: about 35% of activity maximum, pH 11: about 25% of activity maximum, oxidase reaction
Gordonia rubripertincta
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
contains 1 mol of flavin adenine dinucleotide per mol of protein
Gordonia rubripertincta
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
contains 1 mol of flavin adenine dinucleotide per mol of protein
Gordonia rubripertincta
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1-Androstene-3,17-dione
-
Gordonia rubripertincta
estrone
-
Gordonia rubripertincta
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0063
-
11beta-hydroxytestosterone
i.e. 4-androstene-11beta,17beta-diol-3-one
Gordonia rubripertincta
0.0064
-
11-deoxy-17alpha-hydroxycorticosterone
i.e. 4-pregnene-17alpha,21-diol-3,20-dione
Gordonia rubripertincta
0.0066
-
androsterone
i.e. 4-androstene-3,11,17-trione
Gordonia rubripertincta
0.0083
-
19-nortestosterone
4-estren-17beta-ol-3one
Gordonia rubripertincta
0.0089
-
19-nor-4-androstene-3,17-dione
i.e. 4-estren-3,17-dione
Gordonia rubripertincta
0.0143
-
testololactone
i.e. 17alpha-oxo-D-homo-1,4-androstadiene-3,17-dione
Gordonia rubripertincta
0.0196
-
11alpha-hydroxyprogesterone
i.e. 4-pregnene-11alpha-ol-3,20-dione, 11beta-hydroxy-4-androstene-3,17-dione, 4-estren-3,17-dione
Gordonia rubripertincta
0.0204
-
corticosterone
i.e. 4-pregnene-11beta,21-diol-3,20-dione
Gordonia rubripertincta
0.021
-
4-androstene-3,17-dione
-
Gordonia rubripertincta
0.0256
-
cortisone
i.e. 4-pregnene-17alpha,21diol-3,11,20-trione
Gordonia rubripertincta
0.0385
-
hydrocorticosterone
i.e. 4-pregnene-11beta,17alpha,21-triol-3,20-dione
Gordonia rubripertincta
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60500
-
SDS-PAGE, gel filtration
Gordonia rubripertincta
Purification (Commentary) (protein specific)
Commentary
Organism
-
Gordonia rubripertincta
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
113
-
-
Gordonia rubripertincta
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 1 week, stable
Gordonia rubripertincta
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11-deoxy-17alpha-hydroxycorticosterone + acceptor
4-pregnene-17alpha,21-diol-3,20-dione
391375
Gordonia rubripertincta
17,21-dihydroxypregna-1,4-diene-3,20-dione + reduced acceptor
-
-
-
?
11alpha-hydroxyprogesterone + acceptor
4-pregnene-11alpha-ol-3,20-dione
391375
Gordonia rubripertincta
11alpha-hydroxy-1,4-pregnene-3,20-dione + reduced acceptor
-
-
-
?
11beta-hydroxy-4-androstene-3,17-dione + acceptor
-
391375
Gordonia rubripertincta
11beta-hydroxy-1,4-androstene-3,17-dione + reduced acceptor
-
-
-
?
11beta-hydroxytestosterone + acceptor
4-androstene-11beta,17beta-diol-3-one
391375
Gordonia rubripertincta
11beta,17beta-dihydroxyandrost-1,4-diene-3-one + reduced acceptor
-
-
-
?
19-nor-4-androstene-3,17-dione + acceptor
4-estren-3,17-dione
391375
Gordonia rubripertincta
19-nor-1,4-androstene-3,17-dione + reduced acceptor
-
-
-
?
19-nortestosterone + acceptor
4-estren-17beta-ol-3-one
391375
Gordonia rubripertincta
17beta-hydroxy-1,4-estrene-3-one + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
-
391375
Gordonia rubripertincta
1,4-androstene-3,17-dione + reduced acceptor
-
-
-
?
androsterone + acceptor
4-androstene-3,11,17-trione
391375
Gordonia rubripertincta
3-alpha-hydroxy-1-androstene-17-one + reduced acceptor
-
-
-
?
corticosterone + acceptor
4-pregnene-11beta,21-diol-3,20-dione
391375
Gordonia rubripertincta
1,4-pregnene-17alpha,21-diol-3,20-dione + reduced acceptor
-
-
-
?
cortisone + acceptor
4-pregnene-17alpha,21-diol-3,11,20-trione
391375
Gordonia rubripertincta
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor
-
-
-
?
hydrocorticosterone + acceptor
4-pregnene-11beta,17alpha,21triol-3,20-dione
391375
Gordonia rubripertincta
1,4-pregnene-11beta,17alpha,21-triol-3,20-dione + reduced acceptor
-
-
-
?
additional information
acceptor: phenazine methosulfate
391375
Gordonia rubripertincta
?
-
-
-
-
additional information
acceptor: O2
391375
Gordonia rubripertincta
?
-
-
-
-
additional information
not: 3beta-hydroxysteroid, no electron acceptor: NADP+, K3Fe(CN)6, cytochrome c, 3-ketosteroids with a 11alpha- or 11beta-hydroxyl group are oxidized at slow rate, catalyzes aromatization of the A-ring of 19-nortestosterone and 19-norandrostenedione to produce estradiol and estrone, 19-hydroxytestosterone, 19-hydroxyandrosterone, 19-hydroxyandrostenedione and 19-oxotestosterone are converted to the respective phenolic steroids with cleavage of the C10 side-chain
391375
Gordonia rubripertincta
?
-
-
-
-
testololactone + acceptor
D-homo-17a-oxoandrost-4-ene-3,17-dione
391375
Gordonia rubripertincta
D-homo-17alpha-oxoandrosta-1,4-diene-3,17-one + reduced acceptor
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 60500, SDS-PAGE
Gordonia rubripertincta
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
activity increases linearly up to 45°C
Gordonia rubripertincta
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
20 min
Gordonia rubripertincta
52
-
20 min, 75% loss of activity
Gordonia rubripertincta
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
10
oxidase reaction
Gordonia rubripertincta
10
-
dehydrogenation, phenazine methosulfate
Gordonia rubripertincta
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
10.5
pH 7: about 60% of activity maximum, pH 10.5: about 85% of activity maximum, phenazine methosulfate dehydrogenation
Gordonia rubripertincta
8
11
pH 8: about 35% of activity maximum, pH 11: about 25% of activity maximum, oxidase reaction
Gordonia rubripertincta
Other publictions for EC 1.3.99.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743236
Guevara
Functional differentiation of ...
Rhodococcus ruber, Rhodococcus ruber Chol-4
Microb. Cell Fact.
16
42
2017
-
-
-
-
-
-
-
20
-
-
-
-
-
7
-
-
-
-
-
-
-
-
33
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
33
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
743031
Shao
A mutant form of 3-ketosteroi ...
Mycobacterium neoaurum, Mycobacterium neoaurum JC-12, Mycobacterium neoaurum ST-095
J. Ind. Microbiol. Biotechnol.
43
691-701
2016
-
-
1
-
1
-
-
2
-
-
-
-
-
4
-
-
-
-
-
-
1
-
3
1
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
3
1
-
-
-
2
-
-
-
-
-
-
-
-
2
2
743221
Yao
Characterization and engineer ...
Mycobacterium neoaurum, Mycobacterium neoaurum ATCC 25795
Metab. Eng.
24
181-191
2014
-
1
1
-
-
-
-
14
-
-
-
-
-
6
-
-
-
-
-
-
-
-
28
1
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
28
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724670
Brzezinska
The role of 3-ketosteroid 1(2) ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
BMC Microbiol.
13
43
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
151
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
725830
Zhang
Bioconversion of 4-androstene- ...
Mycobacterium neoaurum, Mycobacterium neoaurum JC-12
J. Steroid Biochem. Mol. Biol.
135
36-42
2013
-
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
1
-
4
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
4
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
742850
Rohman
Crystal structure and site-di ...
Rhodococcus erythropolis, Rhodococcus erythropolis SQ1
J. Biol. Chem.
288
35559-35568
2013
-
-
1
1
3
-
-
3
-
-
-
-
-
4
-
-
-
-
-
-
1
-
6
-
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
6
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3
723868
Rohman
Purification, crystallization ...
Rhodococcus erythropolis, Rhodococcus erythropolis SQ1
Acta Crystallogr. Sect. F
68
551-556
2012
-
-
1
1
-
-
-
-
-
-
1
2
-
4
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
1
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
1
-
-
1
1
-
-
-
724074
Chen
Characterization and applicati ...
Aspergillus fumigatus, Aspergillus fumigatus CICC 40167
Appl. Microbiol. Biotechnol.
96
133-142
2012
-
1
1
-
-
-
-
-
1
-
1
2
-
2
-
-
1
-
-
-
-
-
2
1
1
-
-
-
1
-
-
2
-
1
-
-
1
1
2
-
-
-
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A new bacterial steroid degrad ...
Comamonas testosteroni, Comamonas testosteroni TA441
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Pseudomonas fluorescens
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van der Geize
Molecular and functional chara ...
Rhodococcus erythropolis, Rhodococcus erythropolis SQ1
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8
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1
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391391
Van der Geize
Unmarked gene deletion mutagen ...
Rhodococcus erythropolis
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2001
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1
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391394
Fujii
Essential tyrosine residues in ...
Rhodococcus rhodochrous
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1999
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Morii
3-Ketosteroid-DELTA1-dehydroge ...
Rhodococcus rhodochrous
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391392
Choi
Purification and characterizat ...
Pimelobacter simplex
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1995
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5
1
1
1
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391393
Choi
Secretory overproduction of Ar ...
Pimelobacter simplex
Appl. Microbiol. Biotechnol.
43
1044-1049
1995
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391395
Molnar
Molecular cloning, expression ...
Pimelobacter simplex
Mol. Microbiol.
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1995
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391375
Itagaki
Purification and characterizat ...
Gordonia rubripertincta
Biochim. Biophys. Acta
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60-67
1990
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391378
Itagaki
Spectral properties of 3-ketos ...
Gordonia rubripertincta
Biochim. Biophys. Acta
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281-286
1990
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391379
Kloosterman IV
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Effect of supersaturated aqueo ...
Pimelobacter simplex
Enzyme Microb. Technol.
6
113-116
1984
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-
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-
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391385
Udvardy
-
Kinetics of the formation of s ...
Pimelobacter simplex
FEMS Symp.
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1982
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391384
Atrat
Steroid transformation with im ...
Mycobacterium phlei, Nocardia erythropolis, Rhodococcus opacus
Z. Allg. Mikrobiol.
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1981
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Atrat
-
Efficient purification of a mi ...
Rhodococcus opacus
J. Chromatogr.
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279-283
1980
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Hörhold
Steroid transforming enzymes i ...
Rhodococcus opacus
Z. Allg. Mikrobiol.
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1980
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Groh
Steroid-transforming enzymes f ...
Rhodococcus opacus
Z. Allg. Mikrobiol.
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727-730
1979
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2
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-
-
-
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391388
Hörhold
Steroid-transforming enzymes f ...
Rhodococcus opacus
Z. Allg. Mikrobiol.
19
731-739
1979
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-
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Lestrovaja
Steroid transforming enzymes f ...
Rhodococcus opacus
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189-196
1978
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391390
Abul-Hajj
Isolation of vitamin K2(35) fr ...
Ilyonectria destructans, Pimelobacter simplex, Plectosphaerella cucumerina, Rhodococcus hoagii
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253
2356-2360
1978
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15
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391386
Hörhold
Steroid-transforming enzymes f ...
Rhodococcus opacus
Z. Allg. Mikrobiol.
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559-561
1976
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1
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391380
Penasse
The active center of the 3-oxo ...
Pimelobacter simplex
Eur. J. Biochem.
47
555-559
1974
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1
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1
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1
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1
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391382
Rodig
The stereochemistry of hydroge ...
Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
56
467-470
1974
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1
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391381
Aries
-
Degradation of steroids by int ...
Clostridium paraputrificum
Biochim. Biophys. Acta
248
482-488
1971
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5
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1
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391383
Levy
Bacterial oxidation of steroid ...
Comamonas testosteroni
J. Biol. Chem.
234
2014-2021
1959
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