Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-Androstene-3,17-dione | - |
Gordonia rubripertincta | |
estrone | - |
Gordonia rubripertincta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0063 | - |
11beta-hydroxytestosterone | i.e. 4-androstene-11beta,17beta-diol-3-one | Gordonia rubripertincta | |
0.0064 | - |
11-deoxy-17alpha-hydroxycorticosterone | i.e. 4-pregnene-17alpha,21-diol-3,20-dione | Gordonia rubripertincta | |
0.0066 | - |
androsterone | i.e. 4-androstene-3,11,17-trione | Gordonia rubripertincta | |
0.0083 | - |
19-nortestosterone | 4-estren-17beta-ol-3one | Gordonia rubripertincta | |
0.0089 | - |
19-nor-4-androstene-3,17-dione | i.e. 4-estren-3,17-dione | Gordonia rubripertincta | |
0.0143 | - |
testololactone | i.e. 17alpha-oxo-D-homo-1,4-androstadiene-3,17-dione | Gordonia rubripertincta | |
0.0196 | - |
11alpha-hydroxyprogesterone | i.e. 4-pregnene-11alpha-ol-3,20-dione, 11beta-hydroxy-4-androstene-3,17-dione, 4-estren-3,17-dione | Gordonia rubripertincta | |
0.0204 | - |
corticosterone | i.e. 4-pregnene-11beta,21-diol-3,20-dione | Gordonia rubripertincta | |
0.021 | - |
4-androstene-3,17-dione | - |
Gordonia rubripertincta | |
0.0256 | - |
cortisone | i.e. 4-pregnene-17alpha,21diol-3,11,20-trione | Gordonia rubripertincta | |
0.0385 | - |
hydrocorticosterone | i.e. 4-pregnene-11beta,17alpha,21-triol-3,20-dione | Gordonia rubripertincta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60500 | - |
SDS-PAGE, gel filtration | Gordonia rubripertincta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gordonia rubripertincta | - |
inducible | - |
Purification (Comment) | Organism |
---|---|
- |
Gordonia rubripertincta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
113 | - |
- |
Gordonia rubripertincta |
Storage Stability | Organism |
---|---|
4°C, 1 week, stable | Gordonia rubripertincta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxy-17alpha-hydroxycorticosterone + acceptor | 4-pregnene-17alpha,21-diol-3,20-dione | Gordonia rubripertincta | 17,21-dihydroxypregna-1,4-diene-3,20-dione + reduced acceptor | - |
? | |
11alpha-hydroxyprogesterone + acceptor | 4-pregnene-11alpha-ol-3,20-dione | Gordonia rubripertincta | 11alpha-hydroxy-1,4-pregnene-3,20-dione + reduced acceptor | - |
? | |
11beta-hydroxy-4-androstene-3,17-dione + acceptor | - |
Gordonia rubripertincta | 11beta-hydroxy-1,4-androstene-3,17-dione + reduced acceptor | - |
? | |
11beta-hydroxytestosterone + acceptor | 4-androstene-11beta,17beta-diol-3-one | Gordonia rubripertincta | 11beta,17beta-dihydroxyandrost-1,4-diene-3-one + reduced acceptor | - |
? | |
19-nor-4-androstene-3,17-dione + acceptor | 4-estren-3,17-dione | Gordonia rubripertincta | 19-nor-1,4-androstene-3,17-dione + reduced acceptor | - |
? | |
19-nortestosterone + acceptor | 4-estren-17beta-ol-3-one | Gordonia rubripertincta | 17beta-hydroxy-1,4-estrene-3-one + reduced acceptor | - |
? | |
4-androstene-3,17-dione + acceptor | - |
Gordonia rubripertincta | 1,4-androstene-3,17-dione + reduced acceptor | - |
? | |
androsterone + acceptor | 4-androstene-3,11,17-trione | Gordonia rubripertincta | 3-alpha-hydroxy-1-androstene-17-one + reduced acceptor | - |
? | |
corticosterone + acceptor | 4-pregnene-11beta,21-diol-3,20-dione | Gordonia rubripertincta | 1,4-pregnene-17alpha,21-diol-3,20-dione + reduced acceptor | - |
? | |
cortisone + acceptor | 4-pregnene-17alpha,21-diol-3,11,20-trione | Gordonia rubripertincta | 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor | - |
? | |
hydrocorticosterone + acceptor | 4-pregnene-11beta,17alpha,21triol-3,20-dione | Gordonia rubripertincta | 1,4-pregnene-11beta,17alpha,21-triol-3,20-dione + reduced acceptor | - |
? | |
additional information | acceptor: phenazine methosulfate | Gordonia rubripertincta | ? | - |
? | |
additional information | acceptor: O2 | Gordonia rubripertincta | ? | - |
? | |
additional information | not: 3beta-hydroxysteroid, no electron acceptor: NADP+, K3Fe(CN)6, cytochrome c, 3-ketosteroids with a 11alpha- or 11beta-hydroxyl group are oxidized at slow rate, catalyzes aromatization of the A-ring of 19-nortestosterone and 19-norandrostenedione to produce estradiol and estrone, 19-hydroxytestosterone, 19-hydroxyandrosterone, 19-hydroxyandrostenedione and 19-oxotestosterone are converted to the respective phenolic steroids with cleavage of the C10 side-chain | Gordonia rubripertincta | ? | - |
? | |
testololactone + acceptor | D-homo-17a-oxoandrost-4-ene-3,17-dione | Gordonia rubripertincta | D-homo-17alpha-oxoandrosta-1,4-diene-3,17-one + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60500, SDS-PAGE | Gordonia rubripertincta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
activity increases linearly up to 45°C | Gordonia rubripertincta |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
20 min | Gordonia rubripertincta |
52 | - |
20 min, 75% loss of activity | Gordonia rubripertincta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10 | oxidase reaction | Gordonia rubripertincta |
10 | - |
dehydrogenation, phenazine methosulfate | Gordonia rubripertincta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10.5 | pH 7: about 60% of activity maximum, pH 10.5: about 85% of activity maximum, phenazine methosulfate dehydrogenation | Gordonia rubripertincta |
8 | 11 | pH 8: about 35% of activity maximum, pH 11: about 25% of activity maximum, oxidase reaction | Gordonia rubripertincta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 1 mol of flavin adenine dinucleotide per mol of protein | Gordonia rubripertincta |