Application | Comment | Organism |
---|---|---|
synthesis | engineered enzyme mutants, in which KsdDM is inactivated or augmented, are useful for production of phytosterols 4-androstene-3,17-dione or 1,4-androstadiene-3,17-dione, circumventing the difficulty of separating 4-androstene-3,17-dione from 1,4-androstadiene-3,17-dione, a key bottleneck to the microbial transformation of phytosterols in industry | Mycolicibacterium neoaurum |
Cloned (Comment) | Organism |
---|---|
gene kstD, DNA and amino acid sequence determination and analysis, phylogenetic tree, heterologous expression of the His-tagged enzyme, by low-temperature induction at 22°C, the recombinant protein appears partly as soluble protein | Mycolicibacterium neoaurum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androstene-3,17-dione + FAD | Mycolicibacterium neoaurum | - |
1,4-androstadiene-3,17-dione + FADH2 | - |
? | |
4-androstene-3,17-dione + FAD | Mycolicibacterium neoaurum NwIB-01 | - |
1,4-androstadiene-3,17-dione + FADH2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium neoaurum | C6KID9 | - |
- |
Mycolicibacterium neoaurum NwIB-01 | C6KID9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme by nickel affinity chromatography | Mycolicibacterium neoaurum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.8 | - |
soluble recombinant His-tagged enzyme, cell extract, pH 7.0, 30°C | Mycolicibacterium neoaurum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androstene-3,17-dione + FAD | - |
Mycolicibacterium neoaurum | 1,4-androstadiene-3,17-dione + FADH2 | - |
? | |
4-androstene-3,17-dione + FAD | with artificial electron acceptors phenazine methosulfate and dichlorophenolindophenol | Mycolicibacterium neoaurum | 1,4-androstadiene-3,17-dione + FADH2 | - |
? | |
4-androstene-3,17-dione + FAD | - |
Mycolicibacterium neoaurum NwIB-01 | 1,4-androstadiene-3,17-dione + FADH2 | - |
? | |
4-androstene-3,17-dione + FAD | with artificial electron acceptors phenazine methosulfate and dichlorophenolindophenol | Mycolicibacterium neoaurum NwIB-01 | 1,4-androstadiene-3,17-dione + FADH2 | - |
? | |
9alpha-hydroxy-4-androstene-3,17-dione + FAD | - |
Mycolicibacterium neoaurum | 9alpha-hydroxy-1,4-androstadiene-3,17-dione + FADH2 | - |
? | |
9alpha-hydroxy-4-androstene-3,17-dione + FAD | - |
Mycolicibacterium neoaurum NwIB-01 | 9alpha-hydroxy-1,4-androstadiene-3,17-dione + FADH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-ketosteroid-DELTA1-dehydrogenase | - |
Mycolicibacterium neoaurum |
KsdDM | - |
Mycolicibacterium neoaurum |
steroid-1-dehydrogenase | - |
Mycolicibacterium neoaurum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mycolicibacterium neoaurum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mycolicibacterium neoaurum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | putative N-terminal FAD-binding motif in KsdDM is GSG(A/G)(A/G)(A/G)X17E | Mycolicibacterium neoaurum |