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Literature summary for 1.3.99.4 extracted from

  • Guevara, G.; Fernandez de Las Heras, L.; Perera, J.; Navarro Llorens, J.M.
    Functional differentiation of 3-ketosteroid Delta(1)-dehydrogenase isozymes in Rhodococcus ruber strain Chol-4 (2017), Microb. Cell Fact., 16, 42 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.99.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0216
-
 deoxycorticosterone pH 7.0, 30°C Rhodococcus ruber
0.0221
-
 9alpha-hydroxy-4-androstene-3,17-dione pH 7.0, 30°C Rhodococcus ruber
0.0246
-
 (5alpha,17beta)-17-hydroxyandrostan-3-one pH 7.0, 30°C Rhodococcus ruber
0.0271
-
 progesterone pH 7.0, 30°C Rhodococcus ruber
0.0288
-
 testosterone pH 7.0, 30°C Rhodococcus ruber
0.0338
-
 progesterone pH 7.0, 30°C Rhodococcus ruber
0.0342
-
 4-androstene-3,17-dione pH 7.0, 30°C Rhodococcus ruber
0.0382
-
 4-pregnen-3-one-20beta-carboxylic acid pH 7.0, 30°C Rhodococcus ruber
0.0401
-
 4-androstene-3,17-dione pH 7.0, 30°C Rhodococcus ruber
0.0425
-
 deoxycorticosterone pH 7.0, 30°C Rhodococcus ruber
0.0436
-
 progesterone pH 7.0, 30°C Rhodococcus ruber
0.0761
-
 4-pregnen-3-one-20beta-carboxylic acid pH 7.0, 30°C Rhodococcus ruber
0.1079
-
 testosterone pH 7.0, 30°C Rhodococcus ruber
0.1113
-
 deoxycorticosterone pH 7.0, 30°C Rhodococcus ruber
0.1619
-
 corticosterone pH 7.0, 30°C Rhodococcus ruber
0.1811
-
 testosterone pH 7.0, 30°C Rhodococcus ruber
0.3474
-
 19-hydroxy-4-androstene-3,17-dione pH 7.0, 30°C Rhodococcus ruber
0.3688
-
 19-hydroxy-4-androstene-3,17-dione pH 7.0, 30°C Rhodococcus ruber
0.3743
-
 corticosterone pH 7.0, 30°C Rhodococcus ruber
0.5432
-
 9alpha-hydroxy-4-androstene-3,17-dione pH 7.0, 30°C Rhodococcus ruber

Organism

Organism UniProt Comment Textmining
Rhodococcus ruber C6GCU2 isoform KstD3
-
Rhodococcus ruber I0B6I6 isoform KstD2
-
Rhodococcus ruber I0B6J0 isoform KstD1
-
Rhodococcus ruber Chol-4 C6GCU2 isoform KstD3
-
Rhodococcus ruber Chol-4 I0B6I6 isoform KstD2
-
Rhodococcus ruber Chol-4 I0B6J0 isoform KstD1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5alpha,17beta)-17-hydroxyandrostan-3-one + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
(5alpha,17beta)-17-hydroxyandrostan-3-one + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber Chol-4 ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
19-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber 19-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
19-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber Chol-4 19-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber 1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber Chol-4 1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
4-pregnen-3-one-20beta-carboxylic acid + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber pregna-1,4-dien-3-one-20beta-carboxylic acid + reduced 2,6-dichlorophenol-indophenol
-
 ?
4-pregnen-3-one-20beta-carboxylic acid + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber Chol-4 pregna-1,4-dien-3-one-20beta-carboxylic acid + reduced 2,6-dichlorophenol-indophenol
-
 ?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber 9alpha-hydroxyandrost-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber Chol-4 9alpha-hydroxyandrost-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
corticosterone + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber 1,4-pregnene-17alpha,21-diol-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
deoxycorticosterone + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber 1,4-pregnadiene-21-ol-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
deoxycorticosterone + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber 1,4-pregnene-21-ol-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
deoxycorticosterone + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber Chol-4 1,4-pregnene-21-ol-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
progesterone + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
progesterone + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber Chol-4 pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
testosterone + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber 17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol
-
 ?
testosterone + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
testosterone + 2,6-dichlorophenol-indophenol
-
 Rhodococcus ruber Chol-4 ? + reduced 2,6-dichlorophenol-indophenol
-
 ?

Subunits

Subunits Comment Organism
? x * 54800,SDS-PAGE Rhodococcus ruber
? x * 60800,SDS-PAGE Rhodococcus ruber
? x * 61800,SDS-PAGE Rhodococcus ruber

Synonyms

Synonyms Comment Organism
KSTD1
-
 Rhodococcus ruber
KSTD2
-
 Rhodococcus ruber
KSTD3
-
 Rhodococcus ruber