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show all sequences of 1.3.99.8

Purification and properties of 2-furoyl-coenzyme A hydroxylase from Pseudomonas putida F2

Kitcher, J.P.; Trudgill, P.W.; Rees, J.S.; Biochem. J. 130, 121-132 (1972)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Bathocuproin
-
Pseudomonas putida
Cuprizone
-
Pseudomonas putida
cyanide
not
Pseudomonas putida
additional information
not inhibitory: diethyldithiocarbamate, p-chloromercuribenzoate, arsenite, iodoacetamide, 2-n-heptyl-4-hydroxyquinoline N-oxide, azide, alpha,alpha'-bipyridyl, Tiron (i.e. disodium 1,2-dihydroxybenzene-3,5-disulfonate), 8-hydroxyquinoline, menadione
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
0.05
2-Furoyl-CoA
-
Pseudomonas putida
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
copper
-
Pseudomonas putida
additional information
no Fe
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3270000
-
sedimentation equilibrium
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-furoyl-CoA + H2O + acceptor
Pseudomonas putida
-
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
Pseudomonas putida
?
2-furoyl-CoA + H2O + acceptor
Pseudomonas putida F2
-
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
Pseudomonas putida F2
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida F2
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas putida
Reaction
Reaction
Commentary
Organism
2-furoyl-CoA + H2O + acceptor = S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
mechanism
Pseudomonas putida
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-furoyl-CoA + H2O + acceptor
-
391425
Pseudomonas putida
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
391425
Pseudomonas putida
?
2-furoyl-CoA + H2O + acceptor
acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida
391425
Pseudomonas putida
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
product is tautomerized to 5-oxo-DELTA2-dihydro-2-furoyl-CoA
391425
Pseudomonas putida
?
2-furoyl-CoA + H2O + acceptor
-
391425
Pseudomonas putida F2
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
391425
Pseudomonas putida F2
?
2-furoyl-CoA + H2O + acceptor
acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida
391425
Pseudomonas putida F2
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
product is tautomerized to 5-oxo-DELTA2-dihydro-2-furoyl-CoA
391425
Pseudomonas putida F2
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9.5
2-furoyl-CoA + methylene blue
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Bathocuproin
-
Pseudomonas putida
Cuprizone
-
Pseudomonas putida
cyanide
not
Pseudomonas putida
additional information
not inhibitory: diethyldithiocarbamate, p-chloromercuribenzoate, arsenite, iodoacetamide, 2-n-heptyl-4-hydroxyquinoline N-oxide, azide, alpha,alpha'-bipyridyl, Tiron (i.e. disodium 1,2-dihydroxybenzene-3,5-disulfonate), 8-hydroxyquinoline, menadione
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
0.05
2-Furoyl-CoA
-
Pseudomonas putida
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
copper
-
Pseudomonas putida
additional information
no Fe
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3270000
-
sedimentation equilibrium
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-furoyl-CoA + H2O + acceptor
Pseudomonas putida
-
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
Pseudomonas putida
?
2-furoyl-CoA + H2O + acceptor
Pseudomonas putida F2
-
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
Pseudomonas putida F2
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-furoyl-CoA + H2O + acceptor
-
391425
Pseudomonas putida
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
391425
Pseudomonas putida
?
2-furoyl-CoA + H2O + acceptor
acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida
391425
Pseudomonas putida
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
product is tautomerized to 5-oxo-DELTA2-dihydro-2-furoyl-CoA
391425
Pseudomonas putida
?
2-furoyl-CoA + H2O + acceptor
-
391425
Pseudomonas putida F2
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
391425
Pseudomonas putida F2
?
2-furoyl-CoA + H2O + acceptor
acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida
391425
Pseudomonas putida F2
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
product is tautomerized to 5-oxo-DELTA2-dihydro-2-furoyl-CoA
391425
Pseudomonas putida F2
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9.5
2-furoyl-CoA + methylene blue
Pseudomonas putida
Other publictions for EC 1.3.99.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
391427
Koenig
Xanthine dehydrogenase and 2-f ...
Pseudomonas putida, Pseudomonas putida Fu1
J. Bacteriol.
172
5999-6009
1990
-
-
-
-
-
-
-
1
-
-
3
-
-
5
-
-
1
-
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
772
Koenig
Molybdenum involvement in aero ...
Pseudomonas putida, Pseudomonas putida Fu1
Appl. Environ. Microbiol.
55
1829-1834
1989
-
-
-
-
-
-
2
1
-
1
1
2
-
7
-
-
-
-
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
1
1
2
-
-
-
-
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391423
Nagel
-
Bactopterin as component of eu ...
Pseudomonas putida, Pseudomonas putida Fu1
FEMS Microbiol. Lett.
60
323-326
1989
-
-
-
-
-
-
-
-
-
1
-
2
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391425
Kitcher
Purification and properties of ...
Pseudomonas putida, Pseudomonas putida F2
Biochem. J.
130
121-132
1972
-
-
-
-
-
-
4
1
-
2
1
2
-
3
-
-
1
1
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
2
1
2
-
-
-
1
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391426
Griffin
-
Some studies on the purified 2 ...
Pseudomonas putida, Pseudomonas putida F2
Biochem. J.
125
19
1971
-
-
-
-
-
-
-
1
-
-
1
2
-
3
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-