BRENDA - Enzyme Database show
show all sequences of 1.3.99.8

Xanthine dehydrogenase and 2-furoyl-coenzyme A dehydrogenase from Pseudomonas putida Fu1: two molybdenum-containing dehydrogenases of novel structural composition

Koenig, K.; Andreesen, J.R.; J. Bacteriol. 172, 5999-6009 (1990)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
0.05
2-Furoyl-CoA
-
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
alpha2,beta2, 2 * 25000 + 2 * 55000, SDS-PAGE
Pseudomonas putida
55000
-
alpha2,beta2, 2 * 25000 + 2 * 55000, SDS-PAGE
Pseudomonas putida
150000
-
PAGE, gel filtration
Pseudomonas putida
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida Fu1
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas putida
Storage Stability
Storage Stability
Organism
-20°C, stable over a long period of time
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-furoyl-CoA + H2O + acceptor
2-furoyl-CoA is the only substrate
391427
Pseudomonas putida
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
2-furoyl-CoA + H2O + acceptor
2-furoyl-CoA is the only substrate
391427
Pseudomonas putida Fu1
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterotetramer
alpha2,beta2, 2 * 25000 + 2 * 55000, SDS-PAGE
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
0.05
2-Furoyl-CoA
-
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
alpha2,beta2, 2 * 25000 + 2 * 55000, SDS-PAGE
Pseudomonas putida
55000
-
alpha2,beta2, 2 * 25000 + 2 * 55000, SDS-PAGE
Pseudomonas putida
150000
-
PAGE, gel filtration
Pseudomonas putida
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas putida
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable over a long period of time
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-furoyl-CoA + H2O + acceptor
2-furoyl-CoA is the only substrate
391427
Pseudomonas putida
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
2-furoyl-CoA + H2O + acceptor
2-furoyl-CoA is the only substrate
391427
Pseudomonas putida Fu1
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterotetramer
alpha2,beta2, 2 * 25000 + 2 * 55000, SDS-PAGE
Pseudomonas putida
Other publictions for EC 1.3.99.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
391427
Koenig
Xanthine dehydrogenase and 2-f ...
Pseudomonas putida, Pseudomonas putida Fu1
J. Bacteriol.
172
5999-6009
1990
-
-
-
-
-
-
-
1
-
-
3
-
-
5
-
-
1
-
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
772
Koenig
Molybdenum involvement in aero ...
Pseudomonas putida, Pseudomonas putida Fu1
Appl. Environ. Microbiol.
55
1829-1834
1989
-
-
-
-
-
-
2
1
-
1
1
2
-
7
-
-
-
-
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
1
1
2
-
-
-
-
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391423
Nagel
-
Bactopterin as component of eu ...
Pseudomonas putida, Pseudomonas putida Fu1
FEMS Microbiol. Lett.
60
323-326
1989
-
-
-
-
-
-
-
-
-
1
-
2
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391425
Kitcher
Purification and properties of ...
Pseudomonas putida, Pseudomonas putida F2
Biochem. J.
130
121-132
1972
-
-
-
-
-
-
4
1
-
2
1
2
-
3
-
-
1
1
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
2
1
2
-
-
-
1
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391426
Griffin
-
Some studies on the purified 2 ...
Pseudomonas putida, Pseudomonas putida F2
Biochem. J.
125
19
1971
-
-
-
-
-
-
-
1
-
-
1
2
-
3
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
2
-
-
-
1
-
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-
-
4
-
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