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Literature summary for 1.3.99.8 extracted from

  • Koenig, K.; Andreesen, J.R.
    Molybdenum involvement in aerobic degradation of 2-furoic acid by Pseudomonas putida Fu1 (1989), Appl. Environ. Microbiol., 55, 1829-1834.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
cyanide
-
Pseudomonas putida
tungstate
-
Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02 0.05 2-Furoyl-CoA
-
Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum
-
Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-furoyl-CoA + H2O + acceptor Pseudomonas putida
-
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
?
2-furoyl-CoA + H2O + acceptor Pseudomonas putida Fu1
-
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida Fu1
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
0.09
-
micromol 2-furoyl-CoA/min/mg, in crude extract Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-furoyl-CoA + H2O + acceptor
-
Pseudomonas putida S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
?
2-furoyl-CoA + H2O + acceptor acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida Pseudomonas putida S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
?
2-furoyl-CoA + H2O + acceptor
-
Pseudomonas putida Fu1 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
?
2-furoyl-CoA + H2O + acceptor acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida Pseudomonas putida Fu1 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
2-furoyl-CoA + nitro blue tetrazolium Pseudomonas putida