BRENDA - Enzyme Database show
show all sequences of 1.3.99.8

Molybdenum involvement in aerobic degradation of 2-furoic acid by Pseudomonas putida Fu1

Koenig, K.; Andreesen, J.R.; Appl. Environ. Microbiol. 55, 1829-1834 (1989)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
cyanide
-
Pseudomonas putida
tungstate
-
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
0.05
2-Furoyl-CoA
-
Pseudomonas putida
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Molybdenum
-
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
100000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-furoyl-CoA + H2O + acceptor
Pseudomonas putida
-
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
Pseudomonas putida
?
2-furoyl-CoA + H2O + acceptor
Pseudomonas putida Fu1
-
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
Pseudomonas putida Fu1
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida Fu1
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.09
-
micromol 2-furoyl-CoA/min/mg, in crude extract
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-furoyl-CoA + H2O + acceptor
-
772
Pseudomonas putida
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
772
Pseudomonas putida
?
2-furoyl-CoA + H2O + acceptor
acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida
772
Pseudomonas putida
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
772
Pseudomonas putida
?
2-furoyl-CoA + H2O + acceptor
-
772
Pseudomonas putida Fu1
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
772
Pseudomonas putida Fu1
?
2-furoyl-CoA + H2O + acceptor
acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida
772
Pseudomonas putida Fu1
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
772
Pseudomonas putida Fu1
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
2-furoyl-CoA + nitro blue tetrazolium
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cyanide
-
Pseudomonas putida
tungstate
-
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
0.05
2-Furoyl-CoA
-
Pseudomonas putida
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Molybdenum
-
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
100000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-furoyl-CoA + H2O + acceptor
Pseudomonas putida
-
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
Pseudomonas putida
?
2-furoyl-CoA + H2O + acceptor
Pseudomonas putida Fu1
-
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
Pseudomonas putida Fu1
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.09
-
micromol 2-furoyl-CoA/min/mg, in crude extract
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-furoyl-CoA + H2O + acceptor
-
772
Pseudomonas putida
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
772
Pseudomonas putida
?
2-furoyl-CoA + H2O + acceptor
acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida
772
Pseudomonas putida
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
772
Pseudomonas putida
?
2-furoyl-CoA + H2O + acceptor
-
772
Pseudomonas putida Fu1
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
772
Pseudomonas putida Fu1
?
2-furoyl-CoA + H2O + acceptor
acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida
772
Pseudomonas putida Fu1
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
772
Pseudomonas putida Fu1
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
2-furoyl-CoA + nitro blue tetrazolium
Pseudomonas putida
Other publictions for EC 1.3.99.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
391427
Koenig
Xanthine dehydrogenase and 2-f ...
Pseudomonas putida, Pseudomonas putida Fu1
J. Bacteriol.
172
5999-6009
1990
-
-
-
-
-
-
-
1
-
-
3
-
-
5
-
-
1
-
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
772
Koenig
Molybdenum involvement in aero ...
Pseudomonas putida, Pseudomonas putida Fu1
Appl. Environ. Microbiol.
55
1829-1834
1989
-
-
-
-
-
-
2
1
-
1
1
2
-
7
-
-
-
-
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
1
1
2
-
-
-
-
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391423
Nagel
-
Bactopterin as component of eu ...
Pseudomonas putida, Pseudomonas putida Fu1
FEMS Microbiol. Lett.
60
323-326
1989
-
-
-
-
-
-
-
-
-
1
-
2
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391425
Kitcher
Purification and properties of ...
Pseudomonas putida, Pseudomonas putida F2
Biochem. J.
130
121-132
1972
-
-
-
-
-
-
4
1
-
2
1
2
-
3
-
-
1
1
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
2
1
2
-
-
-
1
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391426
Griffin
-
Some studies on the purified 2 ...
Pseudomonas putida, Pseudomonas putida F2
Biochem. J.
125
19
1971
-
-
-
-
-
-
-
1
-
-
1
2
-
3
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-