Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoCl2 | - |
Priestia megaterium | |
CuSO4 | - |
Priestia megaterium | |
HgCl2 | - |
Priestia megaterium | |
pyruvate | substrate inhibition | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
NADH | - |
Priestia megaterium | |
0.55 | - |
pyruvate | - |
Priestia megaterium | |
235 | - |
NH4+ | - |
Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Priestia megaterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Priestia megaterium |
11.2 | - |
- |
Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADH | 11.7% of the activity with pyruvate | Priestia megaterium | L-Gln + NAD+ + H2O | - |
? | |
3-bromopyruvate + NH3 + NADH | 5.3% of the activity with pyruvate | Priestia megaterium | 2-amino-3-bromopropanoate + NAD+ + H2O | - |
? | |
4-methyl-2-oxopentanoate + NH3 + NADH | 1.6% of the activity with pyruvate | Priestia megaterium | 2-amino-4-methylpentanoate + NAD+ + H2O | - |
? | |
D-Ala + H2O + NAD+ | 32.3% of the activity with L-Ala | Priestia megaterium | pyruvate + NH3 + NADH | - |
? | |
D-Ser + H2O + NAD+ | 1.3% of the activity with L-Ser | Priestia megaterium | 3-hydroxypyruvate + NH3 + NADH | - |
? | |
glyoxylate + NH3 + NADH | 6.2% of the activity with pyruvate | Priestia megaterium | aminoacetate + NAD+ + H2O | - |
? | |
L-Ala + H2O + NAD+ | - |
Priestia megaterium | pyruvate + NH3 + NADH | - |
r | |
L-Ser + H2O + NAD+ | 7.9% of the activity with L-Ala | Priestia megaterium | 3-hydroxypyruvate + NH3 + NADH | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
- |
Priestia megaterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 7.5, half-life: 22 days | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
reductive amination | Priestia megaterium |
10.5 | - |
deamination of L-Ala | Priestia megaterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Priestia megaterium | |
NADH | - |
Priestia megaterium |