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Literature summary for 1.4.1.1 extracted from

  • Muresan, L.; Vancea, D.; Presecan, E.; Porumb, H.; Lascu, I.; Oarga, M.; Matinca, D.; Abrudan, I.; Barzu, O.
    Catalytic properties of Sepharose-bound L-alanine dehydrogenase from Bacillus cereus (1983), Biochim. Biophys. Acta, 742, 617-622.
    View publication on PubMed
Search for more literature for 1.4.1.1

General Stability

General Stability Organism
urea, above 3 M, denaturation of soluble enzyme, resistance to denaturation increases by immobilization, 5 M, 30 min, 85% loss of activity of matrix-bound enzyme Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
 NADH soluble enzyme Bacillus cereus
0.18
-
 NAD+ soluble enzyme Bacillus cereus
0.32
-
 NADH Sepharose-bound enzyme Bacillus cereus
0.48
-
 pyruvate soluble enzyme Bacillus cereus
0.52
-
 pyruvate Sepharose-bound enzyme Bacillus cereus
1.18
-
 NAD+ Sepharose-bound enzyme Bacillus cereus
11.3
-
 L-Ala Sepharose-bound enzyme Bacillus cereus
12.5
-
 L-Ala soluble enzyme Bacillus cereus
22
-
 NH4+ Sepharose-bound enzyme Bacillus cereus
30
-
 NH4+ soluble enzyme Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala + H2O + NAD+
-
 Bacillus cereus pyruvate + NH3 + NADH
-
 r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 28 d, immobilized enzyme retains 85% of its activity in 0.1 M phosphate buffer, pH 8 Bacillus cereus
59
-
 10 min, 50% loss of activity of soluble enzyme Bacillus cereus
65
-
 10 min, complete loss of activity, soluble enzyme Bacillus cereus
67
-
 10 min, 50% loss of activity, immobilized enzyme Bacillus cereus
75
-
 10 min, complete loss of activity, immobilized enzyme Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.5 amination reaction, soluble and Sepharose-bound enzyme Bacillus cereus
10.5 11
-
 Bacillus cereus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 25°C, 24 h, concentration 0.001 mg/ml, stable Bacillus cereus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bacillus cereus
NADH
-
 Bacillus cereus