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Literature summary for 1.4.1.1 extracted from
- Alanine dehydrogenase of the beta-lactam antibiotic producer Streptomyces clavuligerus (1980), Arch. Microbiol., 125, 137-142.
General Stability
| General Stability | Organism |
|---|---|
| 93% loss of activity after dialysis | Streptomyces clavuligerus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxobutanoate | weak | Streptomyces clavuligerus |  |
| 3-hydroxypyruvate | competitive with respect to pyruvate | Streptomyces clavuligerus | |
| Cu2+ | - |
Streptomyces clavuligerus | |
| D-Ala | aminating reaction, competitive with respect to NADH, noncompetitive with respect to NH4+ and pyruvate | Streptomyces clavuligerus | |
| Fe2+ | - |
Streptomyces clavuligerus | |
| glyoxylate | weak | Streptomyces clavuligerus | |
| Hg2+ | - |
Streptomyces clavuligerus | |
| Hydroxypyruvate | - |
Streptomyces clavuligerus | |
| L-Ala | reductive amination of pyruvate | Streptomyces clavuligerus | |
| L-Ser | inhibition of aminating activity | Streptomyces clavuligerus | |
| NADPH | inhibition of reductive amination | Streptomyces clavuligerus | |
| NH4+ | inhibition of aminating activity | Streptomyces clavuligerus | |
| Zn2+ | - |
Streptomyces clavuligerus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
NADH | - |
Streptomyces clavuligerus | |
| 0.5 | - |
pyruvate | - |
Streptomyces clavuligerus | |
| 1.1 | - |
pyruvate | - |
Streptomyces clavuligerus | |
| 9.1 | - |
L-Ala | - |
Streptomyces clavuligerus | |
| 20 | - |
NH4+ | - |
Streptomyces clavuligerus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | - |
sucrose density gradient centrifugation | Streptomyces clavuligerus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces clavuligerus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces clavuligerus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 75% saturated ammonium sulfate suspension, purified enzyme stable for at least 12 months | Streptomyces clavuligerus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxobutanoate + NH3 + NADH | no activity | Streptomyces clavuligerus | 2-aminobutanoate + H2O + NAD+ | - |
? | |
| 2-oxobutanoate + NH3 + NADH | 3.7% of the activity with pyruvate | Streptomyces clavuligerus | 2-aminobutanoate + H2O + NAD+ | - |
? | |
| 3-hydroxypyruvate + NH3 + NADH | 6.9% of the activity with pyruvate | Streptomyces clavuligerus | L-Ser + H2O + NAD+ | - |
? | |
| glyoxylate + NH3 + NADH | 5.6% of the activity with pyruvate | Streptomyces clavuligerus | aminoacetate + NAD+ + H2O | - |
? | |
| L-Ala + H2O + NAD+ | - |
Streptomyces clavuligerus | pyruvate + NH3 + NADH | - |
r | |
| L-Ser + H2O + NAD+ | no activity | Streptomyces clavuligerus | 3-hydroxypyruvate + NH3 + NADH | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | 8.6 | pyruvate amination | Streptomyces clavuligerus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Streptomyces clavuligerus | |
| NADH | - |
Streptomyces clavuligerus | |
| NADP+ | 8% of the activity with NAD+ | Streptomyces clavuligerus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
L-Ala | with respect to NADH | Streptomyces clavuligerus | |
| 2 | - |
L-Ala | with respect to NH4+ | Streptomyces clavuligerus | |
| 3 | - |
L-Ala | with respect to pyruvate | Streptomyces clavuligerus | |
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Release 2023.1 (January 2023)
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