Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Shewanella sp. |
expression in Escherichia coli | Carnobacterium sp. |
General Stability | Organism |
---|---|
enzyme is extremely unstable and easily lysed | Carnobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
NAD+ | - |
Bacillus subtilis | |
0.2 | - |
NAD+ | - |
Carnobacterium sp. | |
0.21 | - |
NAD+ | - |
Vibrio proteolyticus | |
0.24 | - |
NAD+ | - |
Shewanella sp. | |
1.73 | - |
L-Ala | - |
Bacillus subtilis | |
3.8 | - |
L-Ala | - |
Carnobacterium sp. | |
7.6 | - |
L-Ala | - |
Shewanella sp. | |
30 | - |
L-Ala | - |
Vibrio proteolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
6 * 43000, SDS-PAGE | Shewanella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Carnobacterium sp. | - |
- |
- |
Carnobacterium sp. St2 | - |
- |
- |
Geobacillus stearothermophilus | - |
- |
- |
Shewanella sp. | - |
- |
- |
Shewanella sp. Ac10 | - |
- |
- |
Vibrio proteolyticus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Shewanella sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala + H2O + NAD+ | - |
Bacillus subtilis | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Geobacillus stearothermophilus | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Vibrio proteolyticus | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Shewanella sp. | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Carnobacterium sp. | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Carnobacterium sp. St2 | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Shewanella sp. Ac10 | pyruvate + NH3 + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Bacillus subtilis |
hexamer | - |
Geobacillus stearothermophilus |
hexamer | 6 * 43000, SDS-PAGE | Shewanella sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal instability may result from relatively low numbers of salt bridges in the enzymes | Shewanella sp. |
additional information | - |
thermal instability may result from relatively low numbers of salt bridges in the enzymes | Carnobacterium sp. |
41 | - |
30 min, 50% loss of activity | Carnobacterium sp. |
59 | - |
30 min, 50% loss of activity | Shewanella sp. |
63 | - |
- |
Bacillus subtilis |
63 | - |
30 min, 50% loss of activity | Vibrio proteolyticus |
64 | - |
30 min, 50% loss of activity | Bacillus subtilis |
81 | - |
30 min, 50% loss of activity | Geobacillus stearothermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Bacillus subtilis | |
additional information | - |
additional information | - |
Shewanella sp. | |
additional information | - |
additional information | - |
Carnobacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus subtilis | |
NAD+ | - |
Geobacillus stearothermophilus | |
NAD+ | - |
Vibrio proteolyticus | |
NAD+ | - |
Shewanella sp. | |
NAD+ | - |
Carnobacterium sp. | |
NADH | - |
Bacillus subtilis | |
NADH | - |
Geobacillus stearothermophilus | |
NADH | - |
Vibrio proteolyticus | |
NADH | - |
Shewanella sp. | |
NADH | - |
Carnobacterium sp. |